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F8687

Sigma-Aldrich

Fetal Bovine Serum

Australia origin, γ-irradiated, sterile-filtered, suitable for cell culture, suitable for hybridoma

Synonym(s):

Fetal Bovine Serum, FBS, Fetal Bovine Sera, GI FBS, GI FCS, GI Fetal Bovine Sera, GI Fetal Bovine Serum, FCS, sera, serum

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About This Item

MDL number:
MFCD00132239
UNSPSC Code:
12352207
biological source:
bovine fetus
origin:
Australia origin
sterility:
γ-irradiated
sterile-filtered
shipped in:
dry ice

biological source

bovine fetus

Quality Level

500

sterility

γ-irradiated
sterile-filtered

product line

FBS Classic

composition

hemoglobin, ≤0.18 mg/mL

origin

Australia origin

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

impurities

≤5 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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Application

Fetal Bovine Serum has been used in the Roswell Park memorial institute medium-1640 (RPMI-1640) medium to culture myeloma cell lines, RPMI-8226 and U-266. It has also been used as a supplement in Dulbecco′s modified Eagle medium (DMEM) to culture human umbilical vein endothelial cells (HUVECs) and 293 T cells.

Biochem/physiol Actions

Fetal Bovine Serum (FBS) is the commonly used serum to supplement cell culture media due to its high nutritional content. FBS, despite its low protein content, is effective in promoting and maintaining the growth of vertebrate mammalian and insect cells. It can be used as a supplement to culture embryonic stem (ES) cells. FBS can also be used to prepare feeder layers and conditioned media.

Linkage

Learn more information of serum

Analysis Note

  • Endotoxin and hemoglobin tested

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
WXBF3346V
WXBD9363V
WXBD5226V
WXBD7799V
WXBF1246V

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Qingqing Wang et al.
Biochemical and biophysical research communications, 549, 1-7 (2021-03-02)
Ixazomib is a selective, effective, and reversible inhibitor of 20S proteasome and is approved for the treatment of multiple myeloma. Ubiquitin-conjugating enzyme E2 (UBE2K) is involved in the synthesis of K48-linked ubiquitin chains and is the target of certain drugs
Baculoviruses: Expression Vector
Haines FJ, et al.
Encyclopedia of Virology (2008)
Yongpan Huang et al.
Molecular medicine reports, 24(4) (2021-08-10)
Homocysteine (Hcy) was discovered to be an independent risk factor for the development of atherosclerosis (AS). Moreover, endothelial‑mesenchymal transition (EndMT) was found to be one of main mechanisms contributing to the pathogenesis of AS. Salidroside (SAL) has diverse pharmacological activities
Isolation and maintenance of avian ES cells
Petitte JN
Handbook of Stem Cells, 471-477 (2004)
Peng Zhang et al.
International journal of stem cells, 14(2), 229-239 (2021-02-27)
Implantation of bone marrow-derived mesenchymal stem cells (BMSCs) has been recognized as an effective therapy for attenuating acute lung injury (ALI). This study aims to discover microRNA (miRNA)-mediated improvement of BMSCs-based therapeutic effects. Mice were treated with lipopolysaccharide (LPS) for
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Articles

Fetal Bovine Serum (FBS) – What You Need to Know

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

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