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Safety Information

F7942

Sigma-Aldrich

Fetal Bovine Serum

Canada origin, sterile-filtered, γ-irradiated, suitable for cell culture

Synonym(s):

FBS, FCS, sera, serum

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About This Item

MDL number:
MFCD00132239
UNSPSC Code:
12352207
NACRES:
NA.71
biological source:
bovine fetus
origin:
Canada origin
sterility:
γ-irradiated
sterile-filtered
shipped in:
dry ice

biological source

bovine fetus

Quality Level

500

sterility

γ-irradiated
sterile-filtered

product line

FBS Classic

composition

Bovine IgG, ≤1.0 mg/mL
Hemoglobin, ≤20 mg/dL

origin

Canada origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL Endotoxin

shipped in

dry ice

storage temp.

−20°C

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General description

Fetal bovine serum is used as a supplement for in vitro and ex vivo cell, tissue and organ cultures. It has important hormones, vitamins, transport proteins and growth factors.

Application

FBS sourced from Canada is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factors to cells in culture. It was used as a supplement for different cell cultures.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
16C423R1

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?

    Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored  refrigerated at 2-8°C.  If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days.  For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance.  For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C.  For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

J van der Valk et al.
Toxicology in vitro : an international journal published in association with BIBRA, 18(1), 1-12 (2003-11-25)
Fetal bovine serum (FBS) is a common supplement to in vitro culture media. A workshop was organized to discuss whether or not fetuses might suffer when blood is withdrawn, and to discuss serum replacement methods. When bovine fetuses are exposed
Werner E G Müller et al.
Cellular and molecular life sciences : CMLS, 75(1), 21-32 (2017-08-05)
In this study, the effect of inorganic polyphosphate (polyP) on the initial phase of angiogenesis and vascularization was investigated, applying the HUVEC cell tube formation assay. PolyP is a physiological and high energy phosphate polymer which has been proposed to
Alireza Rezapour-Lactoee et al.
Journal of materials science. Materials in medicine, 31(11), 101-101 (2020-11-04)
To boost the healing process in a full-thickness wound, a simple and efficient strategy based on adipose-derived mesenchymal stem cells (ADSCs) transplantation is described in this work. To increase the chance of ADSCs immobilization in the wound bed and prevent
Werner E G Müller et al.
Acta biomaterialia, 118, 233-247 (2020-10-20)
Amorphous Ca-phosphate (ACP) particles stabilized by inorganic polyphosphate (polyP) were prepared by co-precipitation of calcium and phosphate in the presence of polyP (15% [w/w]). These hybrid nanoparticles showed no signs of crystallinity according to X-ray diffraction analysis, in contrast to

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