Free Glycerol Reagent

The Free Glycerol Reagent facilitates the quantitative enzymatic determination of glycerol levels in serum or plasma. Additionally, it may also be used to measure glycerol release from lipolysis in adipose tissue.

Glycerol undergoes phosphorylation through the enzymatic action of glycerol kinase (GK), utilizing adenosine-5′-triphosphate (ATP) to produce glycerol-1-phosphate (G-1-P) and adenosine-5′-diphosphate (ADP). Subsequently, glycerol-1-phosphate (G-1-P) is oxidized by glycerol phosphate oxidase (GPO) to yield dihydroxyacetone phosphate (DAP) and hydrogen peroxide (H2O2). This process involves peroxidase (POD), which catalyzes the coupling of hydrogen peroxide (H2O2) with 4-aminoantipyrine (4-AAP) and sodium N-ethyl-N-(3-sulfopropyl) m-anisidine (ESPA) to generate a quinoneimine dye exhibiting an absorbance maximum at 540 nm.The observed increase in absorbance at 540 nm correlates directly with the concentration of free glycerol present in the sample. This method provides a reliable means for quantifying free glycerol levels, enabling accurate assessment of biochemical samples.

The free glycerol reagent uses coupled enzyme reactions resulting in an increase in absorbance at 540 nm that is directly proportional to the glycerol concentration.

The Free Glycerol Reagent provides a precise method for measuring free endogenous glycerol levels through coupled enzyme reactions, bypassing the need for initial lipase hydrolysis. Unlike triglycerides, which are typically bound to proteins and transported in plasma, free glycerol circulates independently. The innovative approach involves enzymatic or alkaline hydrolysis of triglycerides, liberating glycerol and free fatty acids for accurate measurement.

The Serum Triglyceride Determination Kit (Catalog Number TR0100) offers a comprehensive solution for assessing glycerol, true triglycerides, or total triglycerides in serum or plasma samples. Utilizing lipase enzymatic hydrolysis, triglycerides are converted into glycerol and free fatty acids, enabling precise quantification through coupled enzyme reactions by F6428.

Unlike many commercially available triglyceride reagents, this method distinguishes between endogenous glycerol and glycerol derived from lipase hydrolysis of glycerides. With this advanced technology, researchers can confidently analyze glycerol levels with exceptional accuracy and reliability.

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In addition to kits, the individual reagents and glycerol standard are available separately when fewer reactions are needed.