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Merck
CN

F6136

Anti-Horse Spleen Ferritin antibody produced in rabbit

affinity isolated antibody, lyophilized powder

Synonym(s):

Ferritin Antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
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Product Name

Anti-Horse Spleen Ferritin antibody produced in rabbit, affinity isolated antibody, lyophilized powder

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

horse

technique(s)

immunoelectrophoresis: suitable
immunoelectrophoresis: suitable
indirect ELISA: 1:10,000

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

Gene Information

horse ... Ftl(100051593)

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Application

Anti-Horse Spleen Ferritin antibody was used for immunocytochemistry of rat brain sections at a working antibody dilution of 1:2000 and in mouse brain sections at 1:1000. The antibody was used for immunoblotting using cell lysates of mouse primary erythroblasts and human ovarian carcinoma cells and human bronchial epithelial cells at a working dilution of 1:1000. The antibody is suitable for immunoelectrophoresis.
Horse Spleen Ferritin antibody has been used:
  • immunofluorescence
  • in western blotting
  • in immunohistochemistry

Biochem/physiol Actions

Ferritin iron is oxidized from Fe(II) to Fe(III) in the presence of the enzyme ferroxidase.
The antibody specifically recognizes ferritin from horse spleen.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Ferritin consists of homogenous protein segment called as apoprotein and an iron segment which binds to the apoprotein. It is composed of heavy (H) and light (L) chains.
Ferritin is the storage form of iron in mammals, found mainly in liver, bone marrow and spleen. It is also present in small intestine, placenta, kidney and heart. Horse spleen ferritin is made up of 24 identical subunits. Ferritin of different organs is distinct with different electrophoretic mobility and possible functional differences.
.

Immunogen

Purified horse spleen ferritin

Physical form

Lyophilized from 0.01 M sodium phosphate, 0.015 M sodium chloride, pH 7.2

Preparation Note

Reconstitute with 0.135 M sodium chloride.

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Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

常规特殊物品
常规特殊物品
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Jan A Gorter et al.
Epilepsia, 46(9), 1371-1379 (2005-09-09)
Iron accumulation in the brain has been associated with neurodegenerative disorders, including epilepsy. In our previous SAGE study, we showed that ferritin, an iron-storage protein, was one of the genes (Ferritin-H) that showed overexpression before the chronic epileptic phase. In
Microglial pathology in Down syndrome
Xue Q S and Streit W J
Acta Neuropathologica, 122(4), 455-455 (2011)
New functions for an iron storage protein: the role of ferritin in immunity and autoimmunity
Recalcati S, et al.
Journal of Autoimmunity, 30(1-2), 84-89 (2008)
Transferrin ensures survival of ovarian carcinoma cells when apoptosis is induced by TNF$\alpha$, FasL, TRAIL, or Myc
Fassl S, et al.
Oncogene, 22(51), 8343-8343 (2003)
Structure and physiological functions of ferritin
Granick, S
Physiological Reviews, 31(4), 489-511 (1951)

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