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Merck
CN

F5897

Anti-Mouse IgG (Fc specific)–FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Goat Anti-Mouse IgG (Fc specific)–Fluorescein isothiocyanate

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162628

Key Documents

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Product Name

Anti-Mouse IgG (Fc specific)–FITC antibody produced in goat, affinity isolated antibody, buffered aqueous solution

biological source

goat

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
indirect immunofluorescence: 1:64

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

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Application

Anti-Mouse IgG (Fc specific)−FITC antibody produced in goat has been used in:
  • immunohistochemistry
  • immunofluorescence
  • anti-nuclear antibody assay

Biochem/physiol Actions

Anti-Mouse IgG (Fc specific)-FITC antibody is specific for Fc fragment of all mouse IgG subclasses. It shows no reactivity with mouse IgG Fab fragment, rat IgG or human IgG. Goat anti-Mouse IgG is conjugated to FITC and then purified by gel filtration to remove free FITC.
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

IgG subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids.

Immunogen

Purified mouse IgG

Other Notes

Antibody adsorbed with human IgG and rat serum proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with human or rat samples.

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
0000143765
0000206301
0000206301
0000143765
107M4858V

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IgG subclasses and allotypes: from structure to effector functions
Vidarsson G, et al.
Frontiers in Immunology, 5 (2014)
Two NOD Idd-associated intervals contribute synergistically to the development of autoimmune exocrinopathy (Sjogren's syndrome) on a healthy murine background
Cha S, et al.
Arthritis and Rheumatism, 46(5), 1390-1398 (2002)
Reelin signaling is necessary for a specific step in the migration of hindbrain efferent neurons
Rossel M, et al.
Development, 132(6), 1175-1185 (2005)
JmjC domain-containing protein 6 (Jmjd6) derepresses the transcriptional repressor transcription factor 7-like 1 (Tcf7l1) and is required for body axis patterning during xenopus embryogenesis
Zhang X, et al.
The Journal of Biological Chemistry, 290(33), 20273-20283 (2015)
B cells, antibodies, and more
Hoffman W, et al.
Clinical journal of the American Society of Nephrology : CJASN, 11(1), 137-154 (2016)
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