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F5884

Sigma-Aldrich

α-L-Fucosidase from bovine kidney

ammonium sulfate suspension, ≥2.0 units/mg protein (biuret)

Synonym(s):

α-L-Fucoside fucohydrolase

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About This Item

Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.32

biological source

bovine kidney

form

ammonium sulfate suspension

specific activity

≥2.0 units/mg protein (biuret)

mol wt

210-220 kDa (Subunits: 55-60 kDa)

foreign activity

α-mannosidase and β-galactosidase ≤0.1%
β-N-acetylglucosaminidase ≤0.2%

shipped in

wet ice

storage temp.

2-8°C

General description

α-L-fucosidases is a liposomal enzyme, which belongs to glycosyl hydrolases family.

Application

α-L-fucosidase from bovine kidney has been used:
  • to study their ability to alter primary cell transduction
  • to examine the role of fucose residues of cell surface glycans in adhesion
  • to investigate its role during fertilization

Biochem/physiol Actions

α-L-fucosidases degrades fucose-containing fucoglycoconjugates and breaks fucosidic bonds in oligosaccharides and glycoconjugates. It acts as a tumor marker for hepatic and colorectal cancer. α-L-fucosidase exhibits transglycosylation property.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl α-L-fucoside to p-nitrophenol and L-fucose per min at pH 5.5 at 25°C.

Physical form

Suspension in 3.2 M (NH4)2SO4, 10 mM NaH2PO4 10 mM citrate, pH 6.0

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Novel alpha-l-fucosidases from a soil metagenome for production of fucosylated human milk oligosaccharides
Lezyk M, et al.
PLoS ONE, 11(1), e0147438-e0147438 (2016)
Human alpha-L-fucosidase-1 attenuates the invasive properties of thyroid cancer
Vecchio G, et al.
Testing, 8(16), 27075-27075 (2017)
Directed evolution of the alpha-L-fucosidase from Thermotoga maritima into an alpha-L-transfucosidase
Osanjo G, et al.
Biochemistry, 46(4), 1022-1033 (2007)
Alpha-L-Fucosidase Serves as a Prognostic Indicator for Intrahepatic Cholangiocarcinoma and Inhibits Its Invasion Capacity
Shuang Z, et al.
BioMed Research International, 2018 (2018)
alpha-l-Fucosidase enhances capacitation-associated events in porcine spermatozoa
Romero-Aguirregomezcorta J, et al.
The Veterinary Journal, 203(1), 109-114 (2015)

Articles

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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