F4883
Fibrinogen from human plasma
35-65% protein (≥90% of protein is clottable).
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Synonym(s):
Factor I
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biological source
human plasma
Quality Level
description
essentially plasmin(ogen) free
form
powder
quality
35-65% protein (≥90% of protein is clottable).
mol wt
α-chain 63.5 kDa
β-chain 56 kDa
γ chain 47 kDa (about 4% carbohydrate content)
soluble dimer 340 kDa
concentration
35-65% protein (biuret)
technique(s)
cell culture | mammalian: suitable
solubility
0.9% NaCl: soluble
storage temp.
−20°C
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General description
Fibrinogen was the first blood coagulation factor to be identified, and it is a precursor of fibrin protein. This protein is composed of two chains, each of which is composed of three chains- α, β and γ. These three subunits are coded by three genes located in the human chromosomal locus 4q23-q32, and spans 51kb.
Application
Fibrinogen from human plasma has been used for-
- fibrinogen adsorption assay performed on swCNTs (single-walled carbon nanotubes)
- the characterization of fibrinogen structure in the presence of CNT using CD (Circular dichroism) spectroscopy and temperature scan
- the preparation of RBC suspension obtained from venous blood of antecubital vein, to assess RBC aggregability
- fibrinolysis assay performed on mouse embryonic fibroblasts (MEFs)
- the preparation of fibrinogen-labeled gold (Fgn/Au)
- the quantification of in vitro angiogenesis by human microvascular endothelial cells and
- the assessment of fibrin-induced apoptosis and differentiation-regulation in trophoblast obtained from term human placentas.
Biochem/physiol Actions
Fibrinogen is an acute phase protein that is part of the coagulation cascade of proteins. The end result of the cascade is the production of thrombin that converts fibrinogen to fibrin. Thrombin rapidly proteolyses fibrinogen, releasing fibrinopeptide A. The loss of this small peptide is not sufficient to make the resulting fibrin molecule insoluble, but it tends to form complexes with adjacent fibrin and fibrinogen molecules. Thrombin then cleaves a second peptide, fibrinopeptide B, from fibrin and the fibrin monomers formed then polymerize spontaneously to form an insoluble gel. The polymerized fibrin is held together by noncovalent and electrostatic forces and stabilized by the transamidating enzyme, factor XIIIa, that is produced by the action of thrombin on factor XIII. The insoluble fibrin aggregates (clots) and aggregated platelets then block the damaged blood vessel and prevent further bleeding. The amount of fibrinogen in the plasma can serve as a nonspecific indicator of whether or not an inflammatory process is present in the body. Fibrinogen from any mammalian source will be cleaved by thrombin from any mammalian source.
Fibrinogen is an acute-phase inflammatory protein, which plays an essential role in blood clotting. Coronary heart disease is linked to the plasma levels of fibrinogen in Chinese population, and might have potential as a marker and risk factor for the same. A -455 allele of fibrinogen β (FGB) chain is linked with poor survival in Caucasian women with Finnish stroke. Ischemic stroke preceded by hyperfibrinogenemia has poor outcome, which is independent of the baseline fibrinogen concentration.
Specifications
Source material has tested negative for HIV and HBsAg.
Physical form
Lyophilized powder containing sodium citrate and sodium chloride.
Analysis Note
Protein determined by biuret
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Marta Swarowska et al.
Inflammation, 37(4), 1142-1147 (2014-02-18)
Hyperfibrinogenemia at the beginning of ischemic stroke is associated with poor outcome. We hypothesized that the sustained increase of plasma fibrinogen during stroke predicts outcome independently of baseline fibrinogen concentration. We included 266 patients with first-ever ischemic stroke in whom
Bin Song et al.
International journal of clinical and experimental medicine, 8(8), 13195-13202 (2015-11-10)
Coronary heart disease (CHD) remains the leading causes of death and disability for men and women in most developed countries. It may soon become the leading cause of death in developing countries. Several studies have examined the role of fibrinogen
J G White
The American journal of pathology, 137(4), 989-998 (1990-10-01)
Glycoprotein (GP)IIb-IIIa receptors on human platelets in suspension and after surface activation bind a variety of specific antibodies and ligands and transport them across the outside of the plasma membrane by processes of clustering, patching and capping, and endocytosis to
R G Humphrey et al.
Placenta, 26(6), 491-497 (2005-06-14)
We hypothesized that fibrin enhances apoptosis and modulates differentiation of trophoblast in vitro. Cytotrophoblasts isolated from normal term human placentas were cultured < or =72 h in DMEM-10%-FBS on a fibrin matrix in standard or hypoxic conditions. Trophoblasts were cultured
Sung Jean Park et al.
International journal of nanomedicine, 7, 4325-4333 (2012-08-24)
The conformational changes of plasma protein structures in response to carbon nanotubes are critical for determining the nanotoxicity and blood coagulation effects of carbon nanotubes. In this study, we identified that the functional intensity of carboxyl groups on carbon nanotubes
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