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Merck
CN

F4648

Fast Red TR/Naphthol AS-MX

SIGMAFAST, fluorogenic, tablet

Synonym(s):

4-Chloro-2-methylbenzenediazonium / 3-Hydroxy-2-naphthoic acid 2,4-dimethylanilide phosphate tablets

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About This Item

MDL number:
UNSPSC Code:
12352204
NACRES:
NA.83
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Product Name

SIGMAFAST Fast Red TR/Naphthol AS-MX Tablets, tablet, To prepare 1 mL

Quality Level

form

tablet

solubility

water: 1 tablet/mL, clear to hazy, colorless to faintly yellow

storage temp.

−20°C

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Application

Developed for use as a precipitating substrate for the detection of alkaline phosphatase activity in immunochemistry. Primary uses are in immunohistology, immunoblotting, or dot blotting. SIGMAFAST Fast Red TR/Naphthol AS-MX tablets contain 0.6 mM levamisole to block endogenous alkaline phosphatase activity. SIGMAFAST Fast Red TR/Naphthol AS-MX Tablets require no additional steps or buffers to prepare an active substrate solution.
SIGMAFAST Fast Red TR/Naphthol AS-MX Tablets has been used:
  • in alkaline phosphatase staining in organ sections
  • for staining chondrogenic spheroids in immunohistochemistry studies
  • for the detection of hybridization signals in in situ mRNA hybridization studies of human palatine tonsils sections

Preparation Note

Each tablet dissolved in 1 mL deionized water yields a ready-to-use buffered solution.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Pictograms

CorrosionEnvironment

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1C

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

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