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F4274

Sigma-Aldrich

Fluorescein isothiocyanate isomer I

≥97.5% purity (HPLC), powder

Synonym(s):

FITC, Fluorescein 5-isothiocyanate

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About This Item

Empirical Formula (Hill Notation):
C21H11NO5S
CAS Number:
3326-32-7
Molecular Weight:
389.38
Beilstein:
1407295
EC Number:
222-042-0
MDL number:
MFCD00005063
UNSPSC Code:
12171500
PubChem Substance ID:
24894860
NACRES:
NA.47
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Product Name

Fluorescein isothiocyanate isomer I, ≥97.5% (HPLC)

Quality Level

100

Assay

≥97.5% (HPLC)

form

powder

technique(s)

titration: suitable

color

yellow to very dark yellow

mp

>360 °C (lit.)

solubility

acetone: soluble 10 mg/mL

fluorescence

λex 492 nm; λem 518 nm (green)

application(s)

diagnostic assay manufacturing
hematology
histology

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Milli-Q® IQ Element纯化单元 Produces high-quality Type 1 ultrapure water for trace elemental analysis

ZIQELEMT0

Milli-Q® IQ Element纯化单元

Milli-Q® IQ 7015 超纯和纯水净化系统 Produces ultrapure (Type 1) water and pure (Type 2) water with a production flow rate of 15 L/hr from tap water feed.

ZIQ7015T0C

Milli-Q® IQ 7015 超纯和纯水净化系统

Milli-Q® IX Pure Water System with E-POD® Dispenser input: potable tap water, output: type 2 water (> 5 MΩ·cm), The most advanced pure water system for the production of Elix® quality water at a flow rate of 15 L/h, with E-POD® pure water dispenser.

ZIX7015P0C

Milli-Q® IX Pure Water System with E-POD® Dispenser

Milli-Q® IQ 7005 超纯和纯水净化系统 Produces ultrapure (Type 1) water and pure (Type 2) water with a production flow rate of 5 L/hr from tap water feed.

ZIQ7005T0C

Milli-Q® IQ 7005 超纯和纯水净化系统

output

product water quality: type 1 water (18.2 MΩ·cm)

output

product water quality: type 1 water (18.2 MΩ·cm)
product water quality: type 2 water (> 5 MΩ·cm)

output

product water quality: type 2 water (> 5 MΩ·cm)

output

product water quality: type 2 water (> 5 MΩ·cm)
product water quality: type 1 water (18.2 MΩ·cm)

parameter

1.5 L/min flow rate, 9.1 kg operating weight (19.84 lb)

parameter

≤2 L/min distribution flow rate (Type I), 15 L/hr flow rate (Type II), 30 kg operating weight (66 lb), 300 L/day max. usage

parameter

15 L/hr flow rate, 30.0 kg operating weight (66 lb), 300 L/day max. usage, ~2 L/min distribution flow rate

parameter

≤2 L/min distribution flow rate (Type I), 100 L/day max. usage, 26.0 kg operating weight (57.3 lb), 5 L/hr flow rate (Type II)

input

feed water nature ultrapure water

input

feed water nature potable tap water

input

feed water nature potable tap water

input

feed water nature potable tap water

packaging

pkg of 1 unit

packaging

pkg of 1 unit

packaging

pkg of 1 unit (System + E-POD®)

packaging

pkg of 1 unit

conductivity

0.055 μS/cm (Ultrapure water)

conductivity

0.055 μS/cm at 25 °C (Ultrapure Water), <0.2 μS/cm at 25 °C (Pure Water)

conductivity

0.2 μS/cm at 25 °C (water output)

conductivity

0.055 μS/cm at 25 °C (Ultrapure Water), <0.2 μS/cm at 25 °C (Pure Water)

AC/DC input

100 V / 240 V, 50-60 Hz

AC/DC input

100 V / 230 V AC, 50-60 Hz

AC/DC input

100 V / 240 V, 50-60 Hz

AC/DC input

100 V / 230 V AC, 50-60 Hz

General description

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.[1][2]

Application

Fluorescein isothiocyanate isomer I has been used for the staining of conidia.[3][4]
Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

related product

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCH6569
BCBZ8362
BCBQ3411
BCBQ3411V
BCBK0797V

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PU.1 serves a critical role in the innate defense against Aspergillus fumigatus via dendritic cell-associated C-type lectin receptor-1 and toll-like receptors-2 and 4 in THP-1-derived macrophages.
Liu C
Molecular Medicine Reports, 15, 4084-4084 (2017)
Binding of live conidia of Aspergillus fumigatus activates in vitro-generated human Langerhans cells via a lectin of galactomannan specificity.
Persat F
Clinical and Experimental Immunology, 133, 370-370 (2003)
Handbook of Affinity Chromatography (2005)
Ana Bratuša et al.
Colloids and surfaces. B, Biointerfaces, 181, 561-566 (2019-06-12)
This work describes the derivatization of dextran using N-(tert-butyloxycarbonyl)-S-(trityl)-L-cysteine in the presence of N,N'-carbonyldiimidazole (CDI) as a coupling agent. Homogeneous reactions in dimethyl sulfoxide allowed for an efficient coupling of the amino acid derivative to the polymer backbone. Derivatization was
Hermanson GT
Bioconjugate Techniques (2013)
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