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F4021

Sigma-Aldrich

Follicle Stimulating Hormone from human pituitary

~7,000 IU/mg (powder)

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Synonym(s):
FSH, Follitropin
CAS Number:
9002-68-0
MDL number:
MFCD00131099
UNSPSC Code:
51111800
eCl@ss:
42020504
NACRES:
NA.32

biological source

human pituitary glands

Quality Level

200

sterility

non-sterile

form

powder

specific activity

~7,000 IU/mg (powder)

mol wt

35 kDa

technique(s)

cell culture | mammalian: suitable

impurities

HBsAg, HCV and HIV-1/HIV-2, tested negative

pH

7.4

solubility

water: ~50 g/L

UniProt accession no.

P01225
P23945

shipped in

ambient

storage temp.

−20°C

Gene Information

human ... FSHB(2488) , FSHR(2492)

Related Categories

General description

The α chain of FSH has 89 amino acids and the β chain has 115 amino acids. The α chain is not active. FSH belongs to glycoprotein hormone family. It is secreted by the gonadotropic cells of pituitary gland.
Research area: Cell signaling

Application

Follicle stimulating hormone (FSH) from human pituitary has been used for-
  • in vitro luteinization of Macaque granulosa cells
  • luteal cell proliferation assay
  • culturing of preantral follicle cells obtained from mice
  • in vitro maturation of immature cumulus-oocyte complexes (COCs) obtained from mice and
  • culture of RAW264.7 cell line

Biochem/physiol Actions

FSH (follicle stimulating hormone) in involved in the growth of ovarian follicles and steroidogenesis of ovaries. FSHR (follicle-stimulating hormone receptor) interacts with FSH in the ovaries and determines the responsiveness to this hormone. Depressed levels of FSHR result in poor responsiveness and influence folliculogenesis. In the Caucasian population, certain polymorphisms in the FSHR gene are more commonly found in patients with polycystic ovary syndrome (PCOS). In the male fetuses and neonates, it facilitates the proliferation of Sertoli cells. In pubescent males, it stimulates spermatogonia to undergo mitosis.FSH upon binding to its receptor FSH-R positively regulates G-protein coupled receptors/cyclic adenosine monophosphate (cAMP)/protein kinase A pathways to cascade intracellular signals.
The α-chain is not active; biological specificity is attributed to the β-chain. Induces maturation of Graafian follicles of the ovary; promotes development of germinal cells in males; activates cytosolic tyrosine kinase.

Packaging

Package size based on protein content

Other Notes

Two-chain glycoprotein hormone

Quality

Vial also contains 0.1 mg of salts from 0.05 M phosphate buffer, pH 7.4.

antibody

Product No.
Description
Pricing

F2293

Follicle Stimulating Hormone from porcine pituitary

F4021

Follicle Stimulating Hormone from human pituitary, ~7,000 IU/mg (powder)

F3929

Anti-Follicle-Stimulating Hormone Receptor antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

SAB4501041

Anti-FSHR antibody produced in rabbit, affinity isolated antibody

SAB4501042

Anti-FSHR antibody produced in rabbit, affinity isolated antibody

SAB1304978

ANTI-FSHB/FSH (CENTER) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

HPA069703

Anti-FSHB antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody

APREST92880

PrEST Antigen FSHB, Prestige Antigens™ Powered by Atlas Antibodies, buffered aqueous solution

MABX2031

Anti-FSH (10mg) KC, from mouse

ABS580

Anti-FSH, β-subunit Antibody, serum, from goat

MABX2032

Anti-FSH (10mg) KC, from mouse

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

监管及禁止进口产品

Certificates of Analysis (COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

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  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. For Product F4021, Follicle Stimulating Hormone, do you have information on reconstitution, solution storage and potency?

    Product F4021-1VL is packaged as 50 units/vial. Each vial contains roughly 35-36 milligrams solid. Our solubility testing is done at 50 mg/mL, which yields a slightly hazy solution. It may be more convenient to use units/mL for your experiment as it will be more precise. If you decide to try to weigh out the material, please realize that small quantities can be difficult to weigh accurately.For Product F4021, FSH from human pituitary, we recommend solution storage for up to one month at 2-8°C. Unfortunately, we do not have any Sigma data regarding the stability of the porcine hormone.In the absence of information, we would recommend that solutions of Product F4021 not be frozen. If you do decide to store the product at -20°C we would suggest storing it in single use aliquots to avoid numerous freeze/thaw cycles.For the human FSH product we list an approximate activity of 7000 International Units/milligram. This should provide you with a starting point for a conversion calculation. For the porcine product we do not provide a conversion for units to mass. We provide 50 Armour units per vial. The unit definitions are also slightly different. International units are based on an international standard (IRP-68/140) while Armour units are based on Armour FSH standard G-94. According to the literature, 1 Armour unit can contain 9.4-14.2 international units.

  6. Do you have information on the solution stability of Follicle Stimulating Hormone (FSH) from pituitary?

    For information on the solution stability of FSH (and much more), refer to Chem. Soc. Rev., 2(4), 355-395 (1973).From page 369:  "The stabilities of the most highly purified preparations of certain hormonal glycoproteins have presented some problems, e.g. follicle-stimulating hormone preparations are often found to be stable in frozen solution but lose activity dramatically on concentration and freeze drying." Based on that information, we would recommend that if you need to freeze solutions of FSH, you should flash-freeze the solution (using either liquid nitrogen or a dry ice-alcohol bath), and store the solution in the ultra-cold (-70 or -80°C) freezer. Since FSH does have some solution stability concerns, simply placing the solution in a -20°C freezer without first flash-freezing may reduce the activity.

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Karenne N Fru et al.
Biology of reproduction, 75(4), 568-574 (2006-07-14)
Ovulation and luteal formation in primates are associated with the sustained synthesis of progesterone. The observed high intrafollicular concentrations of progesterone during the periovulatory interval raise the possibility that this steroid serves as a precursor for mineralocorticoids. The aim of
J Wu et al.
Biology of reproduction, 63(1), 320-327 (2000-06-22)
Although it is known that LH receptors are present from the time of thecal differentiation, the role of LH during early follicle development is not yet clear. The effect of LH on preantral follicle development has therefore been investigated in
Y Figenschau et al.
Human reproduction (Oxford, England), 12(3), 523-531 (1997-03-01)
A simplified method for the preparation and long-term cultivation of granulosa-luteal cells in serum-free medium is described. The cells were harvested from women undergoing in-vitro fertilization, enriched by sedimentation and dissociated by enzymatic treatment. We demonstrated, by introducing a synthetic
Mahamud-Ur Rashid et al.
PLoS neglected tropical diseases, 14(6), e0008335-e0008335 (2020-06-09)
Zika virus (ZIKV), a neglected tropical disease until its re-emergence in 2007, causes microcephaly in infants and Guillain-Barré syndrome in adults. Its re-emergence and spread to more than 80 countries led the World Health Organization in 2016 to declare a
Alfredo Ulloa-Aguirre et al.
Endocrinology, 159(8), 3020-3035 (2018-07-10)
FSH is synthesized in the pituitary by gonadotrope cells. By binding to and interacting with its cognate receptor [FSH receptor (FSHR)] in the gonads, this gonadotropin plays a key role in the control of gonadal function and reproduction. Upon activation
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