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biological source
feline articular cartilage (normal)
packaging
pkg of 500,000 cells
manufacturer/tradename
Cell Applications, Inc
growth mode
Adherent
karyotype
2n = 38
morphology
chondrocyte
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
arthritis
shipped in
dry ice
storage temp.
−196°C
General description
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Feline Chondrocytes, CnC, are derived from normal feline articular cartilage where they produce and maintain the extracellular matrix of cartilage. Following monolayer culturing, FC can be grown in alginate microspheres to study chondrocyte proliferation and metabolism in a three-dimensional in vitro system. Alginate microspheres provide more physiological conditions for the chondrocytes that allow them to sustain normal proteoglycans production and retention. Because of the prevalence of degenerative joint disease (DJD) in geriatric cats, FC can be a useful tool for veterinary practice.
Feline Chondrocytes, CnC, are derived from normal feline articular cartilage where they produce and maintain the extracellular matrix of cartilage. Following monolayer culturing, FC can be grown in alginate microspheres to study chondrocyte proliferation and metabolism in a three-dimensional in vitro system. Alginate microspheres provide more physiological conditions for the chondrocytes that allow them to sustain normal proteoglycans production and retention. Because of the prevalence of degenerative joint disease (DJD) in geriatric cats, FC can be a useful tool for veterinary practice.
Cell Line Origin
Cartilage
Application
production and maintenance of extracellular matrix, cartilage, collagen, differentiation and de-differentiation, signal transduction, apoptosis, differentiation, drug screening, gene expression, cytokine production, agarose assays, chondrocyte adhesion to medical implants, scaffolds for cartilage regeneration
Components
Feline Chondryocyte Basal Medium containing 10% FBS & 10% DMSO
Preparation Note
- 1st passage, >500,000 cells in Feline Chondryocyte Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 10 doublings
Subculture Routine
Please refer to the FC Culture Protocol.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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