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Merck
CN

F3378

Fast Blue BB Salt hemi(zinc chloride) salt

Dye content ≥80 %, Powder

Synonym(s):

4-Amino-2,5-diethoxybenzanilide diazotated zinc double salt, 4-Benzoylamino-2,5-diethoxybenzenediazonium chloride hemi(zinc chloride) salt

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About This Item

Linear Formula:
C17H18N3O3Cl · 1/2 ZnCl2
CAS Number:
Molecular Weight:
415.94
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
226-817-4
MDL number:
Colour Index Number:
37175
Beilstein/REAXYS Number:
1845288
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Product Name

Fast Blue BB Salt hemi(zinc chloride) salt, Dye content ≥80 %

InChI key

CMFRFQODFZBKTI-UHFFFAOYSA-L

InChI

1S/2C17H17N3O3.4ClH.Zn/c2*1-3-22-15-11-14(20-18)16(23-4-2)10-13(15)19-17(21)12-8-6-5-7-9-12;;;;;/h2*5-11H,3-4H2,1-2H3;4*1H;/q;;;;;;+2/p-2

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.CCOc1cc([N+]#N)c(OCC)cc1NC(=O)c2ccccc2.CCOc3cc([N+]#N)c(OCC)cc3NC(=O)c4ccccc4

form

powder

composition

Dye content, ≥80%

technique(s)

microbe id | staining: suitable

mp

157 °C

solubility

H2O: 10 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

Quality Level

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Application

Fast Blue BB Salt hemi(zinc chloride) salt has been used for alkaline phosphatase staining in cells.
Fast blue BB salt is suitable for the staining of enterochromaffin. Its applications are seen in enzyme histochemistry by Gomori and are suitable for the histochemical demonstration of beta-glucuronidase activities, and various peptidases and proteases. It is also used in enzyme histochemistry including the demonstration of serine esterase in blood smears and histological sections. Fast blue BB salt plays a vital role as a routine stain to demonstrate alkaline phosphatase labeled antibodies.

General description

Fast Blue BB Salt hemi(zinc chloride) salt, also known as Cl Azoic diazo 20 (Cl 37175) is a diazonium salt that possesses a lipophilic amine lipophilic with an aromatic system of moderate size. It is commercially available as the zinc chloride double salt.

Preparation Note

Purified

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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Enzyme-histochemical method for identification of lymphatic capillaries.
Kato S
Lymphology, 24, 125-125 (1991)
Behavior of ?-tricalcium phosphate granules composed of rod-shaped particles in the rat tibia.
Okumura T
J. Ceram. Soc. Jpn., 119, 101-104 (2011)
Peripheral deletion of autoreactive CD8+ T cells in transgenic mice expressing H-2Kb in the liver.
Bertolino P
European Journal of Immunology, 25, 1932-1932 (1995)
R B Hughes et al.
Journal of forensic sciences, 24(4), 842-846 (1979-10-01)
We are reporting what we believe to be a safe, rapid TLC system and spray for the identification of marihuana. The use of Fast Blue 2B salt greatly enhances the specificity of the TLC analysis of suspected marihuana samples.
T Fujimoto et al.
Histochemistry, 65(3), 217-222 (1980-01-01)
The correlated distribution of intramembranous particles and sulfhydryl groups was examined in normal adult rabbit erythrocyte ghosts. Ghosts were treated to exhibit characteristic patterns of particle distribution and sulfhydryl groups were stained with Fast Blue BBN. It was shown that

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