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F3301

Sigma-Aldrich

Factor Xa chromogenic substrate

solid

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Synonym(s):
CH3OCO-D-CHA-Gly-Arg-pNA-AcOH
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.83

Assay

≥90% (HPLC)

Quality Level

form

solid

mol wt

apparent mol wt 622.7

solubility

methanol: water (1:9): 25 mg/mL

storage temp.

2-8°C

Application

Factor Xa chromogenic substrate has been used in factor Xa protease assay for placenta organelle protein and Prospirobolus joannsi.

Biochem/physiol Actions

Factor Xa chromogenic substrate contains peptide sequence Bz-Ile-Glu-Gly-Arg-pNA. Factor Xa mediates the release of p-NA chromogen post hydrolysis of substrate which is measurable photometrically.

Physical properties

KM for human factor Xa is ~0.8mM

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Probing substrate-induced conformational alterations in adrenoleukodystrophy protein by proteolysis
Guimaraes CP, et al.
Journal of Human Genetics, 50(2), 99-99 (2005)
Joannsin, a novel Kunitz-type FXa inhibitor from the venom of Prospirobolus joannsi
Luan N, et al.
Thrombosis and Haemostasis, 117(06), 1031-1039 (2017)
Pathology consultation on anticoagulation monitoring: factor X-related assays
Wool GD and Lu CM
American Journal of Clinical Pathology, 140(5), 623-634 (2013)
Zilei Duan et al.
Cellular and molecular life sciences : CMLS, 79(6), 309-309 (2022-05-22)
Blood clot formation induced by dysfunctional coagulation is a frequent complication of coronavirus disease 2019 (COVID-19) and a high-risk factor for severe illness and death. Neutrophil extracellular traps (NETs) are implicated in COVID-19-induced immunothrombosis. Furthermore, human cathelicidin, a NET component
Ning Luan et al.
Thrombosis and haemostasis, 117(6), 1031-1039 (2017-03-10)
The repugnatorial glands of millipedes release various defensive chemical secretions. Although varieties of such defensive secretions have been studied, none of them is protein or peptide. Herein, a novel factor Xa (FXa) inhibitor named joannsin was identified and characterised from repugnatorial glands

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