biological source
bovine blood (Fetal)
form
saline suspension
extent of labeling
≥200 μg per mL
technique(s)
affinity chromatography: suitable
matrix
cross-linked 4% beaded agarose
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
suitability
suitable for chromatography
storage temp.
2-8°C
Quality Level
Application
Fetuin-agarose is an agarose conjugate in saline suspension used in affinity chromatography, protein chromatography and specialty resins. Fetuin-agarose columns have been used in studies that inform the detection and quantification of tumor markers.
Physical form
Suspension in 0.5M NaCl containing preservative
Preparation Note
Prepared with Fetuin, F2379
Storage Class
10 - Combustible liquids
wgk
WGK 3
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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M Haidar et al.
Glycoconjugate journal, 11(2), 73-79 (1994-04-01)
Envelope glycoproteins of human immunodeficiency virus (gp120 and gp41) occur as oligomers. Here, we show by gel filtration analysis that gp120 oligomerization in vitro is calcium- and temperature-dependent. Recombinant gp120 (rgp120) species were recovered as monomers at 20 degrees C
M Sherman et al.
The Journal of biological chemistry, 269(50), 31479-31483 (1994-12-16)
During heat shock of Escherichia coli, the expression of the major molecular chaperone, GroEL, increases; in addition, a small fraction of the GroEL becomes phosphorylated (Sherman, M. Yu., and Goldberg, A. L. (1992) Nature 367, 166-1692). This heat shock-induced phosphorylation
A K Satapathy et al.
Immunology, 80(4), 546-552 (1993-12-01)
A cell-ELISA was developed using monolayers of glutaraldehyde-fixed normal as well as Plasmodium berghei-infected mouse erythrocytes for quantification and characterization of anti-erythrocytic autoantibodies in murine malaria. Testing normal (NMS) and peak parasitaemic sera (PPS) on erythrocyte monolayers treated with trypsin
S M Baker et al.
Infection and immunity, 63(10), 3920-3926 (1995-10-01)
The gene products from an 8-kb region adjacent to the 3' end of the ptx operon are required by Bordetella pertussis for the export of pertussis holotoxin. At least one of these gene products (PtlC) is specifically required for the
N Seddiki et al.
Biochimica et biophysica acta, 1340(2), 277-282 (1997-07-18)
A synthetic peptide resembling the C2 region of human immunodeficiency virus type 1 (HIV-1) gp120 (C2-Lai: amino acids (aa) 273-288), inhibited (C50 = 200 microM) gp120 calcium-dependent binding of N-acetyl-beta-D-glucosaminyl and mannosyl residues exposed on natural glycoprotein bovine fetuin whereas
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