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About This Item
Form:
lyophilized powder
Biological source:
spinach (Spinacia oleracea)
biological source
spinach (Spinacia oleracea)
Quality Level
form
lyophilized powder
composition
Ferredoxin, ≥15 wt. %
solubility
water: 2 mg/mL, clear, faintly brown
application(s)
cell analysis
storage temp.
−20°C
Gene Information
spinach ... SOVF(137530)
General description
Ferredoxin I (Fd I) from Spinacia oleracea comprises 97 amino acid and is an iron sulfur cluster (2Fe-2S). It is synthesized in the chloroplast. The structure of Fd I has conserved active site.
Application
Ferredoxin from Spinacia oleracea (spinach) has been used:
- as a substrate for spinach ferredoxin reductase,
- for reconstitution with chlorophyll-A for scanning electron microscope (SEM) dispersive X-ray analyzer (EDX) integrated analysis,
- as a reductant in adenylylsulfate (APS) reductase activity of P. aeruginosa
Biochem/physiol Actions
Ferredoxin is important in the electron transfer cascade required for this nitrogen assimilation. It has been used in studies of assimilation of inorganic nitrogen from nitrite or nitrate in the absence of ammonium.
Ferredoxins are iron-containing proteins that serve as electron-transfer catalysts in photosynthesis and nitrogen fixation.
Packaging
Package sizes are based on ferredoxin content.
Physical form
Contains Tris buffer
Preparation Note
Partially purified by the method of Tagawa and Arnon, Nature, 195, 537 (1962).
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signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
低风险生物材料
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Protocols
Enzymatic Assay of Ferredoxin NADP Reductase - To standardize a procedure for the enzymatic assay of Ferredoxin NADP Reductase (EC.1.18.12).
Keita Miyake et al.
The FEBS journal, 287(18), 4016-4031 (2020-01-30)
Bilin pigments play important roles for both light perception and harvesting in cyanobacteria by binding to cyanobacteriochromes (CBCRs) and phycobilisomes (PBS), respectively. Among various cyanobacteria, Acaryochloris marina MBIC 11017 (A. marina 11017) exceptionally uses chlorophyll d as the main photosynthetic pigment absorbing
Harnessing a P450 fatty acid decarboxylase from Macrococcus caseolyticus for microbial biosynthesis of odd chain terminal alkenes
Lee JW, et al.
Metabolic Engineering Communications, 7, e00076-e00076 (2018)
Growth-promoting effect on iron-sulfur proteins on axenic cultures of Entamoeba dispar
Khalifa SAM, et al.
Parasite (Paris, France), 13(1), 51-58 (2006)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| F3013-10MG | 04061833270974 |
| F3013-1MG | 04061833613481 |
| F3013-5MG | 04061833270981 |
