F2918
Anti-Focal Adhesion Kinase (pp125FAK) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Anti-Focal Adhesion Kinase (pp125FAK), Anti-FAK
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About This Item
UNSPSC Code:
12352203
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biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 125 kDa
species reactivity
rat, bovine, human, mouse
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable using cultured human endothelial cell extract
indirect immunofluorescence: 1:4,000 using cultured human endothelial cells
microarray: suitable
western blot: 1:2,000 using cultured human endothelial cells extract
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... PTK2(5747)
mouse ... Ptk2(14083)
rat ... Ptk2(25614)
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General description
Focal Adhesion Kinase (FAK) is a non-receptor protein tyrosine kinase that is expressed ubiquitously. FAK is the critical in forming cell-substratum adhesions or focal adhesion complexes. FAK reportedly has multiple phosphorylation sites. Autophosphorylation of FAK at Tyr397 creates a binding site on FAK for Src-family kinases and is critical for maximum adhesion and migration responses. The phosphorylation of Tyr407 and Tyr861 is induced during epithelial-mesenchymal transition and further augmented during cell migration. FAK activation couples signal transduction via PI3K, CAS and Src. FAK signaling is involved in multiple functions such as proliferation, migration, survival and angiogenesis. Disruption of FAK signalling results in failure of adhesion to the substratum and anoikis (apoptosis) of epithelial cells which are anchorage dependent. FAK has been observed to be upregulated in human tumors such as neuroblastoma and is related to tumor cell survival and prognosis
Anti-Focal Adhesion Kinase (pp125 FAK) antibody recognizes FAK (Focal Adhesion Kinase) in human endothelial cultured cell line (125 kDa). An additional, weaker, lower molecular weight band may be seen.
Anti-Focal Adhesion Kinase (pp125 FAK) antibody recognizes FAK (Focal Adhesion Kinase) in human endothelial cultured cell line (125 kDa). An additional, weaker, lower molecular weight band may be seen.
Immunogen
synthetic peptide corresponding to C-terminal region amino acids 1039-1052 of human, mouse, or rat focal adhesion kinase (pp125FAK).
Application
Anti-Focal Adhesion Kinase (pp125fak) antibody may be used at a working dilution of 1:2000 for detection by immunoblotting in human endothelial cells. The antibody is suitable for immunofluorescence at a working dilution of 1:4000. The antibody is suitable for immunoprecipitation, immunocytochemistry and protein microarray. The antibody had been used for detection by immunoblotting and immunoprecipitation in human breast cancer cell line, MCF-7.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Boris Vassilev et al.
The American journal of pathology, 185(4), 987-1000 (2015-02-15)
The STARD3 gene belongs to the minimal amplicon in HER2-positive breast cancers and encodes a cholesterol-binding membrane protein. To study how elevated StAR-related lipid transfer protein 3 (StARD3) expression affects breast cancer cells, we generated MCF-7 cells stably overexpressing StARD3-green
Xuelian Tang et al.
Cancer biology & therapy, 5(11), 1474-1480 (2006-10-03)
Herein, we report that marine-derived sulfated polysaccharide (MSP), a new kind of polysaccharide extracted from brown alga, exhibits the anti-migration effect in vitro and potently suppress metastasis of Lewis lung carcinoma in vivo. Adhesion assays demonstrated that MSP inhibits the
Jihe Zhao et al.
Cancer metastasis reviews, 28(1-2), 35-49 (2009-01-27)
Cellular interactions with extracellular matrix play essential roles in tumor initiation, progression and metastasis. Focal adhesion kinase (FAK) is a cytoplasmic tyrosine kinase identified as a key mediator of signaling by integrins, a major family of cell surface receptors for
Serine phosphorylation of paxillin by heregulin-beta1: role of p38 mitogen activated protein kinase.
R Vadlamudi et al.
Oncogene, 18(51), 7253-7264 (1999-12-22)
The mechanisms through which heregulin (HRG) regulates the progression of breast cancer cells to a more invasive phenotype are currently unknown. Recently we have shown that HRG treatment of breast cancer cells leads to the formation of lamellipodia/filopodia, and increased
Shouye Wang et al.
Anti-cancer agents in medicinal chemistry, 11(10), 993-1002 (2011-10-26)
AKT (or protein kinase B) and focal adhesion kinase (FAK) are two important kinases that regulate various cellular functions. Each is overexpressed and/or aberrantly activated in diverse cancers. Several small molecular inhibitors targeting either AKT or FAK are in development
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