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Merck
CN

F2756

Fluorescein di(β-D-galactopyranoside)

Synonym(s):

FDG

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About This Item

Empirical Formula (Hill Notation):
C32H32O15
CAS Number:
17817-20-8
Molecular Weight:
656.59
NACRES:
NC.07
PubChem Substance ID:
24894829
UNSPSC Code:
12352201
MDL number:
MFCD00077344

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InChI

1S/C32H32O15/c33-11-21-23(35)25(37)27(39)30(45-21)42-13-5-7-17-19(9-13)44-20-10-14(43-31-28(40)26(38)24(36)22(12-34)46-31)6-8-18(20)32(17)16-4-2-1-3-15(16)29(41)47-32/h1-10,21-28,30-31,33-40H,11-12H2/t21-,22-,23+,24+,25+,26+,27-,28-,30-,31-/m1/s1

SMILES string

OC[C@H]1O[C@@H](Oc2ccc3c(Oc4cc(O[C@@H]5O[C@H](CO)[C@H](O)[C@H](O)[C@H]5O)ccc4C36OC(=O)c7ccccc67)c2)[C@H](O)[C@@H](O)[C@H]1O

InChI key

ZTOBILYWTYHOJB-WBCGDKOGSA-N

assay

≥98% (HPLC)

form

powder

solubility

methanol: 20 mg/mL, clear, yellow

storage temp.

−20°C

Quality Level

100

Related Categories

General description

Fluorogenic substrate for β-galactosidase

Application

Fluorescein di(β-D-galactopyranoside) (FDG) has been used for the detection of gene expression via FACS (fluorescence-activated cell sorting).
Fluorescein di(β-D-galactopyranoside) has been used as a substrate in β-galactosidase assay.

Biochem/physiol Actions

Fluorescein di (β-D-galactopyranoside) (FDG) results in fluorescence after being hydrolyzed to fluorescein by β-galactosidase. It gives fluorescence proportional to the enzymatic activity. This molecule is mainly required for the detection of gene expression in transfected cells when using β-galactosidase (Lac Z) as a reporter gene.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

涉药品监管产品
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Certificates of Analysis (COA)

Lot/Batch Number
17796LH
117H5116
087K5320
102K4612
095K5304

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Tamio Furuse et al.
Disease models & mechanisms, 12(9) (2019-08-11)
Dysfunction of glucose transporter 1 (GLUT1) proteins causes infantile epilepsy, which is designated as a GLUT1 deficiency syndrome (GLUT1DS; OMIM #606777). Patients with GLUT1DS display varied clinical phenotypes, such as infantile seizures, ataxia, severe mental retardation with learning disabilities, delayed
Chaoqin Shen et al.
Molecular cancer, 19(1), 72-72 (2020-04-05)
Epigenetic alterations are involved in various aspects of colorectal carcinogenesis. N6-methyladenosine (m6A) modifications of RNAs are emerging as a new layer of epigenetic regulation. As the most abundant chemical modification of eukaryotic mRNA, m6A is essential for the regulation of
Use of fluorescein-di-beta-D-galactopyranoside (FDG) and C12-FDG as substrates for beta-galactosidase detection by flow cytometry in animal, bacterial, and yeast cells.
Plovins A
Applied and Environmental Microbiology, 60, 4638-4638 (1994)
Dual Somatostatin Receptor/FDG PET/CT Imaging in Metastatic Neuroendocrine Tumours: Proposal for a Novel Grading Scheme with Prognostic Significance.
David Lh Chan et al.
Theranostics, 7(5), 1149-1158 (2017-04-25)
A fluorimetric method using fluorescein di-?-D-galactopyranoside for quantifying the senescence-associated ?-galactosidase activity in human foreskin fibroblast Hs68 cells.
Yang NC and Hu ML
Analytical Biochemistry, 325, 337-343 (2004)
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