F2303
Anti-FKHR (FOXO1a) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
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About This Item
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biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
human, mouse
technique(s)
western blot: 1:2,500 using human FKHR expressed in transfected COS-7 or 293-T cell extracts.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... FOXO1(2308)
mouse ... Foxo1(56458)
General description
Forkhead box protein O1 (FOXO1a) belongs to the forkhead box transcription factor (FOXO) O class family. It comprises of forkhead domain nuclear localization motif, nuclear export and transactivation motifs. FOXO1 gene is mapped to human chromosome 13q14.11.
Specificity
Anti-FKHR (FOXO1a) recognizes human and mouse FKHR (FOXO1a).
Immunogen
synthetic peptide corresponding to human FKHR (amino acids 636-651).
Application
Anti-FKHR (FOXO1a) antibody produced in rabbit may be used in immunoblotting.
Biochem/physiol Actions
Forkhead family members have been shown to play key regulatory roles in embryonic development, differentiation, apoptosis, and tumorigenesis. Forkhead box protein O1 (FOXO1a) or Forkhead in rhabdomyosarcoma (FKHR) contains three residues, Thr24, Ser256, and Ser319 that lie within consensus sequences for phosphorylation by protein kinase B (PKB). Likewise, induction by insulin-like growth factor (IGF-1) and insulin leads to phosphorylation of one or more of these sites. In addition to the involvement of FKHR in apoptosis, it was shown to be involved in glucose homeostasis, cell cycle regulation, and as a nuclear receptor cofactor. FOXO1a has been identified at chromosomal breakpoints in human tumors especially leukemia and rhabdomyosarcoma. FKHR is downregulated in endometrial carcinoma. Antibodies reacting specifically with FKHR (FOXO1a) may be useful in studying the expression and function of the protein, as well as in correlating its expression pattern with physiological functions or pathological conditions.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2–8 °C for up to one month. For prolonged storage, freeze in working aliquots at 20 °C. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Dominique A Glauser et al.
The Journal of endocrinology, 193(2), 195-207 (2007-05-02)
FOXO transcription factors critically control fundamental cellular processes, including metabolism, cell differentiation, cell cycle arrest, DNA repair, and other reactions to cellular stress. FOXO factors sense the balance between stimuli promoting growth and differentiation versus stress stimuli signaling damage. Integrated
Shiming Peng et al.
Cells, 9(1) (2020-01-16)
FoxO1 is a conserved transcription factor involved in energy metabolism. It is tightly regulated by modifications on its mRNA and protein and responds to environmental nutrient signals. FoxO1 controls the transcription of downstream genes mediating metabolic regulation. Dysfunction of FoxO1
Stephen S Myatt et al.
Cancer research, 70(1), 367-377 (2009-12-24)
Endometrial cancer is the most common malignancy of the lower female reproductive tract. The tumor suppressor FOXO1 is downregulated in endometrial cancer compared with normal endometrium but the underlying mechanisms are not well understood. Using microRNA (miR) target prediction algorithms
Yu Wang et al.
BioMed research international, 2014, 925350-925350 (2014-05-28)
Members of the class O of forkhead box transcription factors (FOXO) have important roles in metabolism, cellular proliferation, stress resistance, and apoptosis. The activity of FOXOs is tightly regulated by posttranslational modification, including phosphorylation, acetylation, and ubiquitylation. Activation of cell
Graham Rena et al.
The EMBO journal, 21(9), 2263-2271 (2002-05-01)
FKHR is phosphorylated by protein kinase B (PKB) at Thr24, Ser256 and Ser319 in response to growth factors, stimulating the nuclear exit and inactivation of this transcription factor. Here we identify two further residues, Ser322 and Ser325, that become phosphorylated
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