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Safety Information

F2012

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)–FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Goat Anti-Mouse IgG (whole molecule)–Fluorescein isothiocyanate

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100 μL
CN¥6,895.36

CN¥6,895.36

Estimated to ship onApril 28, 2025Details

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100 μL
CN¥6,895.36

About This Item

MDL number:
MFCD00162642
UNSPSC Code:
12352203
NACRES:
NA.46

CN¥6,895.36

Estimated to ship onApril 28, 2025Details

Request a Bulk Order

biological source

goat

Quality Level

200

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct immunofluorescence: 1:64 (minimum)

storage temp.

2-8°C

target post-translational modification

unmodified

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Gene Information

human ... MBP(4155)

Gene Information

human ... MYC(4609)
mouse ... Myc(17869)
rat ... Myc(24577)

Gene Information

human ... MDM2(4193)
mouse ... Mdm2(17246)

Gene Information

human ... NEFH(4744)

species reactivity

guinea pig (weakly), mammals

species reactivity

rat (weakly), human, mouse (weakly)

species reactivity

mouse, human

species reactivity

chicken, Xenopus

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

isotype

IgG2b

isotype

IgG1

isotype

IgG2a

isotype

IgG1

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein and a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
The product binds all mouse Igs.

Application

Anti-Mouse IgG (whole molecule)−FITC antibody has been used:
  • in immunofluorescence
  • in immunohistochemistry
  • in immunofluorescence staining
  • in immunofluorescence microscopy
  • in confocal imaging

Anti-Mouse IgG (whole molecule)-FITC antibody produced in goat was used for FACS analysis of DF3 antigen on breast cancer cell lines.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of FITC to Anti-Mouse IgG (whole molecule) antibody allows for the visualization of protein by fluorescent microscopy.

Other Notes

Antibody adsorbed with human serum proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with human samples

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCR4303
    SLCR4303
    0000257146
    SLCQ8115
    0000257146

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    Find documentation for the products that you have recently purchased in the Document Library.

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    L Chen et al.
    The Journal of clinical investigation, 96(6), 2775-2782 (1995-12-01)
    The high molecular weight mucin-like glycoprotein, DF3 (MUC1), is overexpressed in the majority of human breast cancers. Here we demonstrate that replication defective recombinant adenoviral vectors, containing the DF3 promoter (bp -725 to +31), can be used to express beta-galactosidase
    The
    The Laboratory Rat (2005)
    Adhesion and differentiation of human mesenchymal stem cells on plasma-functionalized graphenes with different feeding gases
    Baik K Y, et al.
    Carbon, 77(10), 302-310 (2014)
    Differential cellular effects of electroporation and electrochemotherapy in monolayers of human microvascular endothelial cells
    Meulenberg C J W, et al.
    Testing, 7(12), e52713-e52713 (2012)
    Giuseppe Pani et al.
    PloS one, 11(5), e0155260-e0155260 (2016-05-21)
    During orbital or interplanetary space flights, astronauts are exposed to cosmic radiations and microgravity. However, most earth-based studies on the potential health risks of space conditions have investigated the effects of these two conditions separately. This study aimed at assessing
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