F1680
Anti-Flotillin 2 (C-terminal) antibody produced in rabbit
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
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Synonym(s):
Anti-ECS, Anti-ESA, Anti-Epidermal surface antigen, Anti-FLOT2, Anti-Reggie-1
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biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~48 kDa
species reactivity
human
concentration
~1 mg/mL
technique(s)
indirect immunofluorescence: 5-10 μg/mL using human HEK-293T cells
western blot: 0.5-1 μg/mL using whole extract of human HeLa cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... FLOT2(2319)
mouse ... Flot2(14252)
rat ... Flot2(83764)
Related Categories
General description
Flotillin-2 is a protein encoded by the FLOT2 gene in humans. Flotillin 2 is more widely distributed, but is absent in skeletal muscle. FLOT2, also called reggie-1, belongs to flotillin protein family integral membrane proteins comprising of prohibitin homology (PHB) domain or SPFH domain (stomatin/prohibitin/flotillin/HflK/C domain).
Immunogen
synthetic peptide corresponding to amino acid residues 415-428 of human flotillin 2 with an N-terminal added cysteine, conjugated to KLH. The corresponding sequence differs by 2 amino acids in rat and mouse.
Application
Anti-Flotillin 2 (C-terminal) antibody produced in rabbit has been used in:
- immunofluorescence
- immunoprecipitation
- immunoblotting
Biochem/physiol Actions
Flotillin-2 is a protein encoded by the FLOT2 gene in humans. Flotillins are involved in cell adhesion processes and play a role during breast cancer progression. FLOT2 especially plays a functional role in cell-cell adhesion in non-malignant epithelial cells. It may have an essential function in intestinal physiology, especially in the colon. FLOT2 has been implicated in several signaling pathways in tumor cells and can be used as a prognostic biomarker for breast cancer progression.
The prohibitin homology (PHB) domain constitutes a lipid recognition motif which can target proteins to the plasma membrane. The association of flotillin 2 to the plasma membrane depends on palmitoylation and myristolation in a conserved Cys34. Flotillin 2 overexpression is associated with melanoma progression and transformation of SB2 melanoma cells to a highly metastatic line. It is also associated with expression of the thrombin receptor, protease activated receptor 1 (PAR-1), that is implicated in cell growth and metastasis. Flotillin 2 may be used as a marker protein for lipid raft microdomains.
Target description
Flotillin 2 (C-terminal) encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
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Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Benedikt Beckert et al.
International journal of molecular sciences, 20(13) (2019-06-30)
Pemphigus Vulgaris is an autoimmune disease that results in blister formation in the epidermis and in mucosal tissues due to antibodies recognizing desmosomal cadherins, mainly desmoglein-3 and -1. Studies on the molecular mechanisms of Pemphigus have mainly been carried out
Membrane and raft association of reggie-1/flotillin-2: role of myristoylation, palmitoylation and oligomerization and induction of filopodia by overexpression
Neumann-Giesen C, et al.
The Biochemical Journal, 378(2), 509-518 (2004)
Differences in the expression and distribution of flotillin-2 in chick, mice and human muscle cells
Possidonio ACB, et al.
PLoS ONE, 9(8), e103990-e103990 (2014)
Immortalized Human hTert/KER-CT Keratinocytes a Model System for Research on Desmosomal Adhesion and Pathogenesis of Pemphigus Vulgaris
Beckert B, et al.
International Journal of Molecular Sciences, 20(13), 3113-3113 (2019)
Annika Gauss et al.
International journal of medical sciences, 10(10), 1259-1270 (2013-08-29)
The etiopathogenesis of inflammatory bowel diseases (IBD) remains largely unexplained. Flotillins (flotillin-1 and flotillin-2) are ubiquitous proteins which have been linked to inflammation and regeneration. We hypothesized that alterations in the expression of flotillin-2 in enterocytes may be related to
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