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F1418

Ficoll^® 400

Name: Ficoll<SUP>®</SUP> 400
Brand: SIGMA

BioXtra, Type 400-DL, lyophilized powder

Synonym(s):

Polysucrose 400

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About This Item

CAS Number:

26873-85-8

UNSPSC Code:

12352201

NACRES:

NA.25

MDL number:

MFCD00081599

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Properties

type

Type 400-DL

Quality Level

200

product line

BioXtra

form

lyophilized powder

impurities

≤0.0005% Phosphorus (P), ≤0.1% Insoluble matter

ign. residue

<0.1%

color

white

solubility

water: soluble

anion traces

chloride (Cl^-): ≤0.05%, sulfate (SO₄^2-): ≤0.05%

cation traces

Al: ≤0.0005%, Ca: ≤0.001%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Mg: ≤0.001%, NH₄⁺: ≤0.05%, Na: ≤0.01%, Pb: ≤0.001%, Zn: ≤0.0005%

storage temp.

15-25°C

Description

Application

Ficoll^® 400 is used together with sodium diatrizoate (Paque) to create Ficoll-Paque, a density mixture, used for the separation of cells and organelles, especially lymphocytes and monocytes from blood. Ficoll 400^® may be used for the development of new cell and organelle separation systems. Ficoll 400 is used in tissue engineering and macromolecular conformation research for the development, evaluation and use of macromolecular crowding (MMC) systems and configurations.

Used for cell separation and organelle isolation.

Other Notes

A nonionic synthetic polymer of sucrose.

To gain a comprehensive understanding of our extensive range of Polysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Legal Information

Ficoll is a registered trademark of Cytiva

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Protocols

Ficoll^® 400 for Gradient Centrifugation

Ficoll 400^® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

用于梯度离心的Ficoll® 400

Ficoll 400®是由蔗糖和环氧氯丙烷共聚而成的一种高度分支化的聚合物。Ficoll 400®是完全非离子性的。由于其含有丰富的羟基，Ficoll 400®具有很强的亲水性和水溶性。

Soluble form of the endothelial adhesion molecule CD146 binds preferentially CD16+ monocytes.

Garibaldi S, Barisione C, Ghigliotti G, et al.

Molecular Biology Reports, 39(6), 6745-6752 (2011)

Superiority of visipaque (iodixanol)-controlled density gradient over Ficoll-400 in adult porcine islet purification.

T Min et al.

Transplantation proceedings, 42(5), 1825-1829 (2010-07-14)

Sufficient and favorable biological functions of islets are major problems hindering xenotransplantation. The aim of the present study was to evaluate the effects on harvesting, purity, viability, and function of using improved Visipaque (iodixanol) and Ficoll-400 for adult porcine islet

Selective Expansion of CD34+ Cells from Mouse Bone Marrow Cultured on LH/P MP-Coated Plates with Adequate Cytokines.

Kishimoto S, Ishihara M, Kanatani Y, et al.

Journal of Tissue Engineering, 2, doi: 10-doi: 10 (2011)

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Global Trade Item Number

SKU	GTIN
F1418-25G	04061833620175
F1418-100G	04061833620168