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F1058

Sigma-Aldrich

FSL-Fluorescein

Synonym(s):

FSL-FLRO4(fluorescein)-SA2-L1

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About This Item

Empirical Formula (Hill Notation):
C73H109N4O15PS
Molecular Weight:
1345.70
UNSPSC Code:
41116100
PubChem Substance ID:
329799672
NACRES:
NA.32

Quality Level

200

storage temp.

−20°C

SMILES string

CCCCCCCC\C=C/CCCCCCCC(=O)OCC(COP(O)(=O)OCCNC(=O)CCCCC(=O)NCCCCCNC(=S)Nc1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35)OC(=O)CCCCCCC\C=C/CCCCCCCC

InChI

1S/C73H109N4O15PS/c1-3-5-7-9-11-13-15-17-19-21-23-25-27-29-32-40-69(82)87-55-60(90-70(83)41-33-30-28-26-24-22-20-18-16-14-12-10-8-6-4-2)56-89-93(85,86)88-51-50-75-68(81)39-35-34-38-67(80)74-48-36-31-37-49-76-72(94)77-57-42-45-62-61(52-57)71(84)92-73(62)63-46-43-58(78)53-65(63)91-66-54-59(79)44-47-64(66)73/h17-20,42-47,52-54,60,78-79H,3-16,21-41,48-51,55-56H2,1-2H3,(H,74,80)(H,75,81)(H,85,86)(H2,76,77,94)/b19-17-,20-18-

InChI key

OZXSVOLFZKVFHP-CLFAGFIQSA-N

Application

FSL Constructs Video Protocol
All FSL constructs disperse in biocompatible media and spontaneously and stably incorporate into cell membranes. Cells modified with FSL constructs are known as kodecytes and usually maintain their normal vitality and functionality. FSL-fluorescein has been specifically designed to create fluorescein-labeled live cells (kodecytes) but can also be used to modify other hydrophobic surfaces including fixed cells, virions, and solid phase surfaces. Fluorescein-labeled kodecytes can be visualized at the approximate absorbance wavelength of 488 nm and emission at 518 nm.

Other Notes

FSL-fluorescein is a KODE™ technology construct designed to label hydrophobic surfaces including living cells with biotin. All FSL constructs consist of three essential designable features:
  • functional component (F)
  • spacer (S)
  • diacyl lipid (L)
FSL-fluorescein is comprised of a fluorescein residue representing F, conjugated via a spacer (SA2) to an an activated adipate derivative of dioleoylphosphatidylethanolamine (L1).
Packaged as 1 mg FSL-Fluorescein with 0.42 mg sodium bicarbonate to improve solubility.

Legal Information

KODE Constructs are supplied by KODE Biotech Materials Limited under license from KODE Biotech Limited.
KODE is a trademark of KODE Biotech Ltd.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCF5772
SLCD6702
SLBT4301
SLBJ8140V
SLBF5921V

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What should I use to reconstitute product F1058, FSL-Fluorescein? 

    We recommend reconstituting 1 mg of product F1058, FSL-Fluorescein with 0.5ml of saline or PBS for a concentration of 2 mg/ml.

  6. May I use serum with the product F1058, FSL-Fluorescein?

    We do not recommend diluting the product F1058, FSL-Fluorescein, in lipid-containing buffers. The FSL will associate with the lipids and reduce the efficiency of insertion into cell membranes.

  7. Will the product F1058, FSL-Fluorescein label dead cells?

    The product F1058, FSL-Fluorescein, is designed to insert into the membranes of live cells. But it can also be used to label fixed cells, enveloped virions, and solid phase surfaces.

  8. What concentration of F1058, FSL-Fluorescein do I use to label cells?

    For cell labeling we recommend using a concentration of 10-100ug/ml of F1058, FSL-Fluorescein diluted in PBS. Label cells with FSL-Fluorescein in PBS at a 1:1 volume to volume ratio. The exact concentration may need to be optimized for the cells and the application. Keep incubations in the dark.

  9. How many cells can I label with product F1058, FSL-Fluorescein?

    The exact number of cells may vary depending on cell type and cell condition. The recommended concentration to use for labeling is 10-100ug/ml (~7.4 mM-74mM), which is mixed in a 1:1 volume with cells.

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Victor Naumenko et al.
Molecular therapy oncolytics, 10, 14-27 (2018-08-04)
Oncolytic virus (OV) therapy is an emerging cancer treatment that uses replicating viruses to infect and kill tumor cells and incite anticancer immunity. While the approach shows promise, it currently fails most patients, indicating strategies to improve OV activity are

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