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Merck
CN

F0916

Anti-Fibronectin antibody, Mouse monoclonal

clone IST-4, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Fibronectin antibody produced in mouse, Anti-CIG, Anti-ED-B, Anti-FINC, Anti-FN, Anti-FNZ, Anti-GFND, Anti-GFND2, Anti-LETS, Anti-MSF, Anti-SMDCF

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00162291

Key Documents

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Product Name

Anti-Fibronectin antibody, Mouse monoclonal, clone IST-4, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

IST-4, monoclonal

mol wt

antigen 240 kDa

contains

15 mM sodium azide

species reactivity

human

should not react with

rabbit, mouse, goat, bovine, canine, chicken

technique(s)

immunocytochemistry: 1:100 using cultured human fibroblasts
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
indirect ELISA: suitable
radioimmunoassay: suitable
western blot: 1:500-1000 using human fibroblasts cell extract

isotype

IgG1

UniProt accession no.

P02751

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... FN1(2335)

Related Categories

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Monoclonal Anti-Fibronectin antibody has been used :
  • in immunohistochemistry
  • in confocal microscopy
  • in immunocytochemistry
  • western blotting analysis

Mouse monoclonal clone IST-4 anti-Human Fibronectin antibody may be used for the specific labeling of fibronectin in cultured cells and in frozen tissue sections. Staining of formalin-fixed, paraffin-embedded sections may require prolonged proteolytic unmasking (e.g., by pepsin but not by trypsin).

Biochem/physiol Actions

Fibronectin participates in growth, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in the formation of extracellular matrix.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Fibronectin (FN) is an extracellular matrix protein composed of two nearly-identical disulfide-bound polypeptides with typical molecular weights of 220-280 kDa. Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma and antibodies to either form usually cross-react. Careful analysis of the FN molecule indicates that it contains several functionally and structurally distinct domains which may bind to cell surfaces and to a variety of molecules such as collagen, heparin, gelatin, fibrin and DNA. FN′s play an important role in diverse biological phenomena including cell adhesion, cell migration, hemostasis and thrombosis, wound healing and the ability to induce a more normal phenotype in transformed cells.
Fibronectin 1 is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35.
Mouse monoclonal clone IST-4 anti-Human Fibronectin antibody recognizes an epitope located within the 5th type III repeat of human plasma fibronectin. This epitope is common to all fibronectin forms. The antibody localizes plasma and cellular fibronectin both in its native and denatured-reduced forms (240 kDa) applying immunoblotting, RIA, ELISA, immunocytochemistry and immunohistochemistry. The product reacts with human fibronectin. No reactivity is observed with other species including goat, bovine, dog, rabbit, mouse and chicken.

Immunogen

human plasma fibronectin

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000515767
0000515767
0000466587
0000466587
0000442797

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Clinicopathology of graft detachment after D escemet's membrane endothelial keratoplasty
Brockmann T, et al.
Acta Ophthalmologica, 92(7), e556-e561 (2014)
Francesca Di Modugno et al.
Oncogene, 37(42), 5605-5617 (2018-06-17)
We demonstrated previously that the splicing of the actin regulator, hMENA, generates two alternatively expressed isoforms, hMENA11a and hMENAΔv6, which have opposite functions in cell invasiveness. Their mechanisms of action have remained unclear. Here we report two major findings: (i)
TAZ promotes cell proliferation and epithelial-mesenchymal transition and is inhibited by the hippo pathway
Lei Q Y, et al.
Molecular and cellular biology, 28(7), 2426-2436 (2008)
Shanna A Arnold et al.
Clinical & experimental metastasis, 33(1), 29-44 (2015-10-13)
The extracellular matrix protein fibronectin (FN) contributes to the structural integrity of tissues as well as the adhesive and migratory functions of cells. While FN is abundantly expressed in adult tissues, the expression of several alternatively spliced FN isoforms is
The formation of a functional retinal pigment epithelium occurs on porous polytetrafluoroethylene substrates independently of the surface chemistry
Kearns V R, et al.
Journal of Materials Science. Materials in Medicine, 28(8), 124-124 (2017)
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