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Merck
CN

F0500

Fast Blue RR Salt

crystalline

Synonym(s):

4-Benzoylamino-2,5-dimethoxybenzenediazonium chloride hemi(zinc chloride) salt, Azoic Diazo No. 24

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About This Item

Linear Formula:
C15H14ClN3O3 · 1/2 ZnCl2
CAS Number:
14726-29-5
Molecular Weight:
387.89
NACRES:
NA.47
PubChem Substance ID:
24894722
UNSPSC Code:
12171500
EC Number:
238-777-5
MDL number:
MFCD00074762
Colour Index Number:
37155

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Product Name

Fast Blue RR Salt, crystalline

InChI key

LLARVKAOQLUCQI-UHFFFAOYSA-L

InChI

1S/2C15H13N3O3.4ClH.Zn/c2*1-20-13-9-12(18-16)14(21-2)8-11(13)17-15(19)10-6-4-3-5-7-10;;;;;/h2*3-9H,1-2H3;4*1H;/q;;;;;;+2/p-2

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.COc1cc([N+]#N)c(OC)cc1NC(=O)c2ccccc2.COc3cc([N+]#N)c(OC)cc3NC(=O)c4ccccc4

form

crystalline

technique(s)

microbe id | staining: suitable

solubility

water: 1 mg/mL, clear to hazy, yellow-green

ε (extinction coefficient)

≥15000 at 326-332  nm
≥7000 at 390-396 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

Quality Level

200

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Application

Fast blue RR salt has been used in the immunohistochemistry to detect the activity of alkaline phosphatase. It has been used in the procedure of connective tissue staining using picrosirius red.

Biochem/physiol Actions

Fast blue RR is mainly used in histological stainings. It binds to the reaction product of alkaline phosphatase and precipitates it as an insoluble compound. The sites of alkaline phosphatase activity appears black. Fast blue RR is also used for the detection of esterase activity. It couples with naphthol (released from naphthol acetate) and forms an insoluble azo-dye which appears red/blue.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000381664
0000381664
0000239392
0000239392
SHBP1078

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