EPI001
Histone Acetyltransferase (HAT) Activity Assay Kit
100 assays in 96 well plates
Synonym(s):
HAT Activity, Histone Acetyltransferase Activity
Product Name
Histone Acetyltransferase (HAT) Activity Assay Kit, 100 assays in 96 well plates
usage
100 assays in 96 well plates
shipped in
wet ice
storage temp.
−20°C
Features and Benefits
- Sensitive and reliable assay
- Utilizes colorimetric methods
- Sample type: cell and tissue lysates
- Species reactivity: mammalian
- Suitable for individual tests or high throughput assays and kinetic studies
- Easy and simple procedure: combine nuclear extract or protein sample with reagents, incubate, and read absorbance
- Convenient 96-well microplate format
- Continuously measures HAT activity; suitable for kinetic studies
- No HDAC interference; crude nuclear extract can be used directly
General description
Histone acetyltransferases (HATs) have critical roles in cellular functions and are involved in the regulation of gene transcription, differentiation, and proliferation. The HAT Activity Colorimetric Assay Kit offers a convenient, non-radioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit includes an active Nuclear Extract (NE) to be used as a positive control, HAT cofactor, acetyl-CoA, and all downstream cofactors and substrates. Acetylation of a peptide substrate by active HAT releases the free form of CoA, which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous, and therefore, is suitable for kinetic studies. The kit provides all reagents and a simple, straightforward protocol for a complete assay.
Storage Class
12 - Non Combustible Liquids
Regulatory Information
监管及禁止进口产品
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