E8406
Anti-EDEM1 antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
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Synonym(s):
Anti-EDEM, Anti-ER degradation enhancer, mannosidase alpha-like 1
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 65 kDa (nonglycosylated human and mouse EDEM1)
antigen 75 kDa (glycoslylated human and mouse EDEM1)
species reactivity
human, mouse
technique(s)
immunoprecipitation (IP): 0.5-1.0 μg using extract HEK-293T cells expressing recombinant mouse EDEM1 protein.
western blot: 0.5-1.0 μg/mL using whole extracts of HEK-293T cells expressing recombinant human EDEM1.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... EDEM1(9695)
mouse ... Pvalb(19293)
General description
ER degradation-enhancing alpha-mannosidase-like 1 (EDEM1) is an enzyme encoded by the EDEM1 gene in humans. In mammalian cells, EDEM1 is localized to the endoplasmic reticulum (ER), mainly as a soluble glycoprotein.
Immunogen
synthetic peptide corresponding to amino acids 629-646 of human EDEM1, conjugated to KLH via an N-terminal cysteine residue. The corresponding sequence is identical in mouse EDEM1.
Application
Anti-EDEM1 antibody has been used in immunoblotting.
Anti-EDEM1 antibody produced in rabbit is suitable for the following applications:
- Immunoprecipitation at a concentration of 0.5-1.0μg using extract HEK-293T cells expressing recombinant mouse EDEM1 protein.
- Western blotting at a concentration of 0.5-1.0μg/mL using whole extracts of HEK-293T cells expressing recombinant human EDEM1.
- Immunofluorescence Microscopy.
- Immunoblotting
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Biochem/physiol Actions
ER degradation-enhancing alpha-mannosidase-like 1 (EDEM1), a putative mannose binding lectin, targets misfolded glycoproteins for degradation in an N-glycan dependent manner. It interacts with the C-terminus of calnexin and lacks mannosidase activity.
ER degradation-enhancing alpha-mannosidase-like 1 is an enzyme encoded by the EDEM1 gene in humans. It is a chaperone for rod opsin and its expression can be used to promote correct folding enhancing degradation of mutant proteins in the ER to combat protein-misfolding disease. The endogenous EDEM1 in cells that are not stressed by the expression of a transgenic misfolded protein reaches the cytosol and is degraded by basal autophagy. EDEM may maintain the retrotranslocation competence of NHK by inhibiting aggregation. It is also considered responsible for directing aberrant proteins for ERAD (ER-associated protein degradation).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Redundant and antagonistic roles of XTP3B and OS9 in decoding glycan and non-glycan degrons in ER-associated degradation
van der Goot A T, et al.
Molecular Cell, 70, 516-530 (2018)
EDEM1 recognition and delivery of misfolded proteins to the SEL1L-containing ERAD complex
Cormier J H, et al.
Molecular Cell, 34(5), 627-633 (2009)
Sujin Park et al.
Histochemistry and cell biology, 142(2), 153-169 (2014-03-26)
ER degradation-enhancing α-mannosidase-like 1 protein (EDEM1) is involved in the routing of misfolded glycoproteins for degradation in the cytoplasm. Previously, we reported that EDEM1 leaves the endoplasmic reticulum via non-COPII vesicles (Zuber et al. in Proc Natl Acad Sci USA
Redundant and Antagonistic Roles of XTP3B and OS9 in Decoding Glycan and Non-glycan Degrons in ER-Associated Degradation
van der Goot AT, et al.
Molecular Cell, 70(3), 516-530 e6-516-530 e6 (2018)
Fulvio Reggiori et al.
Cell host & microbe, 7(6), 500-508 (2010-06-15)
Coronaviruses (CoV), including SARS and mouse hepatitis virus (MHV), are enveloped RNA viruses that induce formation of double-membrane vesicles (DMVs) and target their replication and transcription complexes (RTCs) on the DMV-limiting membranes. The DMV biogenesis has been connected with the
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