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E8145

Sigma-Aldrich

Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid tetrasodium salt

≥97%

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Synonym(s):
EGTA tetrasodium, Egtazic acid tetrasodium, Ethylene-bis(oxyethylenenitrilo)tetraacetic acid tetrasodium
Empirical Formula (Hill Notation):
C14H20N2O10Na4
CAS Number:
13368-13-3
Molecular Weight:
468.28
MDL number:
MFCD00274420
UNSPSC Code:
12352202
PubChem Substance ID:
24894667
NACRES:
NA.77

Quality Level

200

Assay

≥97%

technique(s)

RNA purification: suitable

solubility

H2O: soluble 10%, clear, colorless

application(s)

life science and biopharma

SMILES string

[Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CCOCCOCCN(CC([O-])=O)CC([O-])=O)CC([O-])=O

InChI

1S/C14H24N2O10.4Na/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24;;;;/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24);;;;/q;4*+1/p-4

InChI key

BAOGCDPNNOBWQZ-UHFFFAOYSA-J

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Application

A chelating agent useful for the determination of calcium in the presence of magnesium. Repeated washing with EGTA-buffer can experimentally deplete cultured cells of Ca2+.
EGTA is suitable for use:
  • in media for chelating Ca2+ to facilitate efficient incorporation of Sr2+ into the oocytes
  • as a component of phosphatase wash buffer used to wash magnetic beads during dephosphorylation and radiolabeling of RNA segments cross-linked to immunoprecipitated proteins
  • as a component of oocyte activation media

Biochem/physiol Actions

Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetra acetic acid tetrasodium salt (EGTA) dissociates mucin complex and is useful in immunohistochemical studies. It suppresses root growth and mitotic index in germinating wheat seeds. It is used as a decalifying agent in root canal biomechanical preparation and is effective compared to EDTA and cyclohexanediaminetetraacetic acid (CDTA).
Ethylene glycol-bis(ß-aminoethyl ether)-N,N,N,N-tetra acetic acid tetrasodium salt (EGTA) is a calcium chelator that binds to Ca2+ in the presence of Mg2+ at a ratio of 1:1.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sayaka Wakayama et al.
Methods in enzymology, 476, 151-169 (2010-08-10)
Somatic cell nuclear transfer (SCNT) has become a unique and powerful tool for epigenetic reprogramming research and gene manipulation in animals. Although the success rates of somatic cloning have been inefficient and the mechanism of reprogramming is still largely unknown
Shirley P and Saunders T L
Advanced Protocols For Animal Transgenesis, 271-277 (2011)
Schmid, R.W. et al.
Analytical Chemistry, 29, 264-264 (1957)
EGTA treatment of human airways in vitro unmasks M1/MUC5AC mucin in submucosal glands
Roger P, et al.
The European Respiratory Journal, 18(1), 176-183 (2001)
EGTA, a calcium chelator, affects cell cycle and increases DNA methylation in root tips of Triticum aestivum L.
Zhang C, et al.
acta societatis botanicorum poloniae, 85(3) (2016)
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