E7510
Earle′s Balanced Salt Solution 10x
Without sodium bicarbonate, 10 ×, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
Earle’s Balanced Salts, EBSS
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About This Item
Quality Level
sterility
sterile-filtered
form
liquid
concentration
10 ×
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
glucose: 10.0 g/L
NaHCO3: no
phenol red: 0.11 g/L
shipped in
ambient
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General description
Earle′s Balanced Salt Solution 10x is a saline solution with physiological pH. It is useful in inducing autophagy and promotes Bacillus Calmette-Guérin (BCG)-induced dendritic cells (DCs) maturation.
Application
Earle′s Balanced Salt Solution 10x has been used:
- as an additive to the nutritive medium sheep nematode Trichostrongylus colubriformis eggs
- in the swim-up method to prepare human sperm sample
- in the steady-state medium to culture bovine primary mammary epithelial cells
Features and Benefits
- Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance.
- Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism.
- When combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism.
- Provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6).
Reconstitution
Supplement with 2.2 g/L sodium bicarbonate at 1×.
supplement
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Yanli Ren et al.
Cancer letters, 390, 126-136 (2017-01-22)
Dyregulation of autophagy is implicated in human cancers and the mechanism details remains largely unclear. Herein we report the regulatory role of miR-638 in autophagy of esophageal squamous cell carcinoma (ESCC) and breast cancer cells. We found that miR-638 overexpression
Siwen Yang et al.
Food & function, 11(1), 424-434 (2019-12-13)
Insulin resistance is the main cause of type 2 diabetes, fatty liver and obesity. Our previous study found that mushroom polysaccharides improved insulin resistance in vitro, but the underlying mechanisms were still unknown. Thus, we investigate the hypoglycemic effects of
A L Molan et al.
The Veterinary record, 150(3), 65-69 (2002-02-12)
The effects of condensed tannins extracted from seven forages on the viability of the eggs and first stage (L1) larvae of the sheep nematode Trichostrongylus colubriformis were evaluated in in vitro assays. The extracts of condensed tannins were obtained from
Zhenlu Li et al.
Cell death & disease, 9(5), 483-483 (2018-05-01)
Autophagy is an essential cytoprotective response against pathologic stresses that selectively degrades damaged cellular components. Impaired autophagy contributes to organ injury in multiple diseases, including ischemia/reperfusion (I/R), but the exact mechanism by which impaired autophagy is regulated remains unclear. Several
Kinsley Tate et al.
International journal of molecular sciences, 22(19) (2021-10-14)
The developing prenatal brain is particularly susceptible to environmental disturbances. During prenatal brain development, synapses form between neurons, resulting in neural circuits that support complex cognitive functions. In utero exposure to environmental factors such as pharmaceuticals that alter the process
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