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Safety Information

E6779

Millipore

EZview™ Red Anti-HA Affinity Gel

Synonym(s):

Anti-HA

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Select a Size

0.2 MG
¥2,217.29
0.5 MG
¥3,324.19
5 X .2 MG
¥6,970.62

¥2,217.29

Available to ship on2025年4月14日Details

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0.2 MG
¥2,217.29
0.5 MG
¥3,324.19
5 X .2 MG
¥6,970.62

About This Item

UNSPSC Code:
12352203
NACRES:
NA.56

¥2,217.29

Available to ship on2025年4月14日Details

shelf life

1 yr at 2‑8 °C

Quality Level

200

analyte chemical class(es)

proteins (hemagglutinin)

technique(s)

affinity chromatography: suitable
immunoprecipitation (IP): suitable

matrix

crosslinked agarose

capacity

≥0.4 mg/mL, gel binding capacity (HA-tagged fusion protein)

storage temp.

2-8°C

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Hepatocyte Growth Factor human HGF, recombinant, expressed in Baculovirus infected High-5 cells, suitable for cell culture

H1404

Hepatocyte Growth Factor human

EGF human Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

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Epidermal Growth Factor from murine submaxillary gland EGF, suitable for cell culture

E4127

Epidermal Growth Factor from murine submaxillary gland

recombinant

expressed in E. coli

recombinant

expressed in Baculovirus infected High-5 cells

recombinant

expressed in E. coli

recombinant

-

assay

≥97% (SDS-PAGE)

assay

>95% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Quality Level

300

Quality Level

200

Quality Level

-

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

lyophilized

form

lyophilized powder

General description

EZview Red Anti-HA Affinity Gel is a red colored Anti-HA agarose affinity gel that contains anti-HA monoclonal antibody which is covalently attached to crosslinked agarose beads. The HA tag has the sequence YPYDVPDYA and derives from the hemagglutinin (HA) protein. This sequence occurs at amino acid residues 98-106 of the full-length HA protein. The HA tag has a high presence of charged amino acids, which enhances its likelihood of strong antibody recognition.

Specificity

Binding specificity: HA-tag peptide (N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C). It recognizes reduced forms of HA-tagged proteins and is reactive with N or C-terminal HA-tagged fusion proteins.

Application

EZview™ Red Anti-HA Affinity Gel has been used for immunoblot analysis[1] and immunoprecipitation assay.[2]
Suitable for Affinity capture (immunoprecipitation) of N- or C- terminal HA-tagged fusion proteins from E. coli or mammalian cell.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduces the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Features and Benefits

  • Increased visibility - Red color reduces risk of incidental aspiration
  • Improved recovery of target protein by reduced accidental loss
  • Higher reproducibility - More consistent yields

Other Notes

The affinity gel contains affinity purified anti-HA mouse monoclonal antibody (Clone HA-7) covalently attached to beaded agarose. The HA tag corresponds to amino acid residues 98-106 (YPYDVPDYA) of human influenza virus hemagglutinin (HA).

Physical form

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon

Legal Information

EZview is a trademark of Sigma-Aldrich Co. LLC

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000373135
    0000360953
    0000353120
    0000343116
    0000307406

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