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Merck
CN

E6758

Sigma-Aldrich

Ethylenediaminetetraacetic acid

anhydrous, crystalline, BioReagent, suitable for cell culture

Synonym(s):

(Ethylenedinitrilo)tetraacetic acid, EDTA, Edathamil, Ethylenedinitrilotetraacetic acid

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100 G
¥512.23
500 G
¥1,031.59

¥512.23


Estimated to ship on2025年4月27日Details


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100 G
¥512.23
500 G
¥1,031.59

About This Item

Linear Formula:
(HO2CCH2)2NCH2CH2N(CH2CO2H)2
CAS Number:
Molecular Weight:
292.24
Beilstein:
1716295
EC Number:
MDL number:
UNSPSC Code:
12161700
eCl@ss:
39030907
PubChem Substance ID:
NACRES:
NA.25

¥512.23


Estimated to ship on2025年4月27日Details


Request a Bulk Order

grade

anhydrous

Quality Level

product line

BioReagent

Assay

≥98.5%

form

crystalline

reaction suitability

reagent type: chelator

technique(s)

cell culture | mammalian: suitable

pH

2.5 (23 °C, 10 g/L)

mp

250 °C (dec.) (lit.)

solubility

3  M NaOH: 100 mg/mL

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1 of 4

This Item
ED03609RDD017
grade

anhydrous

grade

purified grade

grade

-

grade

ACS reagent

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

cell culture | mammalian: suitable

assay

≥98.5%

assay

≥98.5%

assay

≥99.0% (KT)

assay

99.4-100.6%

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

100

product line

BioReagent

product line

-

product line

BioUltra

product line

Redi-Dri

pH

2.5 (23 °C, 10 g/L)

pH

2.5 (23 °C, 10 g/L)

pH

2.5 (23 °C, 10 g/L)

pH

2.5 (23 °C, 10 g/L)

Application

Ethylenediaminetetraacetic acid has been used:
  • as a component of peptide dilution buffer for intracellular BH3 (Bcl-2 homology (BH) domain) profiling and validation[1]
  • as a medium component in the hepatic stellate cell (HSCs) culture[2]
  • as a component of homogenization buffer for protein extraction from eye tissues[3]
  • for stopping benzonase reaction in lysis buffer[4]

Biochem/physiol Actions

Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, majorly used in preventing clotting of blood samples.[5] EDTA may trigger aggregation of platelets resulting in pseudothrombocytopenia.[6] EDTA along with tetracycline imparts serves as root conditioning agent and provides relief in gum infection.[5] EDTA-tetracycline therapy decreases coronary artery calcium levels in atherosclerosis.[7] Calcium Disodium EDTA administration is effective in slowing down the progression of chronic kidney disease.[8]

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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The blockade of lipophagy pathway is necessary for docosahexaenoic acid to regulate lipid droplet turnover in hepatic stellate cells
Qiu S, et al.
Biomedicine and Pharmacotherapy, 109, 1841-1850 (2019)
Analysis of Direct Interaction between Viral DNA-binding Proteins by Protein Pull-down Co-immunoprecipitation Assay
Lechuga A, et al.
Bio-protocol, 8(1), 1571-1571 (2018)
Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation
Dai J and Luftig MA
Journal of Immunology, ji1701473-ji1701473 (2018)
Protein Expressions of the Small GTPase Rho Proteins in Pterygial Tissue and Leukocytes of Patients with Pterygium
Saracaloglu A, et al.
Proceedings, 2(25), 1571-1571 (2018)
Comparison of the efficacy of ethylenediaminetetraacetic acid and tetracycline hydrochloride as root conditioning agents: An in vitro study
Nanda T, et al.
Journal of the International Clinical Dental Research Organization, 4(1), 2-2 (2012)

Protocols

TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer

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