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E6632

Sigma-Aldrich

Epoxy-activated-Agarose

lyophilized powder

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MDL number:
MFCD00163561
UNSPSC Code:
23151817
NACRES:
NA.56

form

lyophilized powder

Quality Level

200

extent of labeling

≥20 μmol per mL

technique(s)

affinity chromatography: suitable

matrix

cross-linked 4% beaded agarose

matrix active group

epoxy

matrix attachment

through epoxy to hydroxyl

matrix spacer

12 atoms (when ligands are coupled to free epoxy groups. Linkage is a stable, uncharged ether.)

swelling

1 g swells to ~3 mL

suitability

suitable for chromatography

storage temp.

−20°C

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General description

Epoxy-activated-Agarose is a resin. Ligands can be covalently linked to agarose via the interaction of the nucleophilic groups of the ligand with the epoxy group.

Application

Epoxy-activated-Agarose is a lyophilized powder, stabilized with lactose, which is used in affinity chromatography, protein chromatography and activated/functionalized matrices. Epoxy-activated agarose has been used in studies informing anti-proliferative activity on human-derived cancer cells as well as cancer prevention.

Other Notes

Ligand: 1,4-bis(2:3-Epoxypropoxy)butane
Attachment: one epoxy group
Spacer: provides a 12-atom spacer when ligands are coupled to free epoxy groups. Linkage is a stable, uncharged ether.

Physical form

Lyophilized powder stabilized with lactose

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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