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E5630

Sigma-Aldrich

β-Estradiol 6-(O-carboxy­methyl)oxime: BSA

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MDL number:
MFCD03613560
UNSPSC Code:
51111800
NACRES:
NA.77

sterility

non-sterile

Quality Level

200

form

lyophilized powder

extent of labeling

~30 mol steroid per mol BSA

solubility

phosphate buffer: 0.90-1.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow

shipped in

ambient

storage temp.

2-8°C

Related Categories

General description

β-Estradiol 6-(O-carboxy­methyl)oxime: BSA is an estradiol conjugate and is membrane-impermeable. BSA is linked to the estradiol at position 6 to form the β-estradiol 6-(O-carboxymethyl) oxime: BSA conjugate.

Application

β-Estradiol 6-(O-carboxy­methyl)oxime: BSA has been used:
  • to test its effect on the N-methyl-D-aspartate receptor (NMDAR) currents in female dorsal root ganglion (DRG) neurons
  • to coat microplates for the detection of plasma 17β-Estradiol levels using indirect enzyme-linked immunosorbent assay (ELISA)
  • to test its effect on MutL homolog 1 in colorectal cancer lines

Biochem/physiol Actions

β-Estradiol 6-(O-carboxy­methyl)oxime: BSA mimics estrogen and favors amyloid precursor protein α (sAPPα) secretion in vitro studies with primary rat cortical neurons. It also stimulates mitogen-activated protein kinase (MAPK) activity in human umbilical vein endothelial cells

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Warning

Hazard Statements

H302 + H312 + H332,H351

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 2

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the molecular weight of Product E5630, β-Estradiol 6-(O-carboxy­methyl)oxime: BSA?

    The Certificate of Analysis has lot specific information for the number of moles of steroid per mole of bovine serum albumin.  From this data and the molecular weights of the components, an approximate molecular weight for the compound can be calculated.  For example, if the moles of beta estradiol to BSA is 32, then the approximate molecular weight for the compound is 76.4 kDa (BSA has a molecular weight of approximately 66.4 kDa and 32 moles of estradiol is approximately 10 kDa more).

  4. What is the shelf life of Product E5630, β-Estradiol 6-(O-carboxy­methyl)oxime: BSA?

    There is no assigned shelf life date for the product.  The guarantee of quality is one year from the time customer receives the product for unopened bottles stored at 2-8°C.

  5. What is the solubility of Product E5630, β-Estradiol 6-(O-carboxy­methyl)oxime: BSA?

    We have solubilized the product in phosphate buffered saline at 1 mg/mL.  Solutions may be somewhat hazy.

  6. What is the solution stability of Product E5630, β-Estradiol 6-(O-carboxy­methyl)oxime: BSA?

    We have not performed solution stability studies, and have found no information on solution stability in the literature. We would therefore recommend using freshly prepared solutions.

  7. How is Product E5630, β-Estradiol 6-(O-carboxy­methyl)oxime: BSA, prepared?

    Our method of preparation is considered proprietary information  However, a method of preparation (not necessarily Sigma-Aldrich’s) has been described in J. Biol. Chem., 228, 713-727 (1957).

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Adrián Tintos et al.
Ecotoxicology and environmental safety, 66(2), 139-147 (2006-02-10)
To assess the effects of naphthalene on liver intermediary metabolism and plasma steroid hormones, immature female rainbow trout (Oncorhynchus mykiss), in a first experiment, were intraperitoneally injected (2 microLg(-1)) with vegetable oil alone (control) or containing naphthalene (10 and 50
Sex-dependent differences in the activity and modulation of N-methyl-d-aspartic acid receptors in rat dorsal root ganglia neurons.
McRoberts, et al.
Neuroscience, 148, 1015-1020 (2018)
Sun Zhang et al.
Acta pharmacologica Sinica, 26(2), 171-176 (2005-01-25)
To investigate the mechanism of the action of estrogen, which stimulates the release of secreted amyloid precursor protein alpha (sAPP(alpha)) and decreases the generation of amyloid-beta protein (A(beta)), a dominant component in senile plaques in the brains of Alzheimer's disease
K S Russell et al.
Proceedings of the National Academy of Sciences of the United States of America, 97(11), 5930-5935 (2000-05-24)
Estrogen induces both rapid and delayed effects on the cardiovascular system. The early effects take place within minutes (e.g., changes in vasomotor tone) and are mediated through rapid intracellular signaling pathways; whereas the delayed effects (e.g., remodeling or lipid alterations)
Claudio R F Marinho et al.
PloS one, 4(5), e5630-e5630 (2009-05-23)
Pregnancy-associated malaria (PAM) is associated with placenta pathology and poor pregnancy outcome but the mechanisms that control the malaria parasite expansion in pregnancy are still poorly understood and not amenable for study in human subjects. Here, we used a set
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