E4781
Anti-eRF3a/GSPT1 antibody produced in rabbit
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
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Synonym(s):
Anti-Eukaryotic Release Factor 3a, Anti-G1 to S phase transition 1, Anti-GST1, homolog of yeast
Recommended Products
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 76.5 kDa
species reactivity
human
packaging
antibody small pack of 25 μL
concentration
~1 mg/mL
technique(s)
immunoprecipitation (IP): 1-2 μg using HeLa cell lysates
indirect immunofluorescence: 5-10 μg/mL using paraformadehyde-fixed HeLa cells
western blot: 0.5-1 μg/mL using HeLa cell lysates
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... GSPT1(2935)
mouse ... Gspt1(14852)
rat ... Gspt1(24420)
General description
Eukaryotic release factor 3 a (eRF3a) is encoded by the gene G1 to S phase transition 1 (GSPT1) in humans. The eRF3a/GSPT1 exon 1 is a translation termination protein characterized with a tri-nucleotide GGC repeat coding for a polyglycine expansion in the amino-terminal end.
Immunogen
synthetic peptide corresponding to amino acids 190-204 of human eRF3a/GSPT1, conjugated to KLH via a C-terminal added cysteine residue. The sequence is conserved in human, rat, and mouse.
Application
Anti-eRF3a/GSPT1 antibody produced in rabbit has been used in immunoprecipitation, indirect immunofluorescence and western blotting.
Biochem/physiol Actions
Eukaryotic release factor 3 a (eRF3a) is proteolytically processed into an IAP (Inhibitors of Apoptosis Proteins)-binding protein. Processed eRF3a interacts with IAPs and can promote caspase activation, IAP ubiquitination and apoptosis. The IAP binding motif is exposed only after proteolytic cleavage of a 69-residue leader sequence. eRF3a also affects mRNA stability and translation initiation by interacting with Poly(A)-binding protein (PABP) and the surveillance factor Upf1.
Eukaryotic release factor 3a (eRF3a) is a translation termination protein that is encoded by G1 to S phase transition 1 gene (GSPT1) in humans. The eRF3a/GSPT1 exon 1 contains a tri-nucleotide GGC repeat coding for a polyglycine expansion in the N-terminal of the protein. It is associated with protein translation as a regulator of apoptosis signal-regulating kinase 1 (ASK1). GSPT1 interacts with ASK1 and enhances ASK1-induced apoptotic activity by activating caspase-3. It plays a novel role in the regulation of ASK1-mediated apoptosis. GSPT1 is found to be up-regulated in various human cancers and is considered as a potential proto-oncogene.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
常规特殊物品
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GGCn polymorphism of eRF3a/GSPT1 gene and breast cancer susceptibility
Miri M, et al.
Medical Oncology (Northwood, London, England), 29 (2012)
Calpain mediates processing of the translation termination factor eRF3 into the IAP-binding isoform p-eRF3
Hashimoto, Y, et al.
Febs Letters, 589 (2015)
The GTP-binding Release Factor eRF3 as a Key Mediator Coupling Translation Termination to mRNA Decay
Kobayashi T, et al.
The Journal of biological chemistry, 279 (2004)
Joana Malta-Vacas et al.
Cancer genetics and cytogenetics, 195(2), 132-142 (2009-12-08)
The human eukaryotic release factor 3a (eRF3a), encoded by the G1 to S phase transition 1 gene (GSPT1; alias eRF3a), is upregulated in various human cancers. GSPT1 contains a GGC(n) polymorphism in exon 1, encoding a polyglycine expansion in the
Mahboobe Miri et al.
Medical oncology (Northwood, London, England), 29(3), 1581-1585 (2011-11-22)
The significance of translation regulatory factors in elevating the risk of cancer has been recently recognized. Eukaryotic release factor 3a (eRF3a) is a translation termination protein that is encoded by G1 to S phase transition 1 gene (GSPT1). The eRF3a/GSPT1
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