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E4656

Sigma-Aldrich

Anti-eRF3a/GSPT1 (N-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-Eukaryotic Release Factor 3a, Anti-G1 to S phase transition 1, Anti-GST1, homolog of yeast

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About This Item

MDL number:
MFCD09265358
UNSPSC Code:
12352203

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 76.5 kDa

species reactivity

human

technique(s)

immunoprecipitation (IP): 2-5 μL using HeLa cell lysates
western blot: 1:500-1:1,000 using HeLa cell lysates

UniProt accession no.

P15170

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GSPT1(2935)

Immunogen

synthetic peptide corresponding to amino acids 99-116 of human eRF3a/GSPT1, conjugated to KLH via a C-terminal added cysteine residue.

Application

Anti-eRF3a/GSPT1 (N-terminal) antibody produced in rabbit is suitable for immunoprecipitation at a concentration of 2-5μL using HeLa cell lysates and western blotting at a dilution of 1:500-1:1,000 using HeLa cell lysates.

Biochem/physiol Actions

Eukaryotic release factor 3a (eRF3a) is a translation termination protein that is encoded by G1 to S phase transition 1 gene (GSPT1) in humans. The eRF3a/GSPT1 exon 1 contains a tri-nucleotide GGC repeat coding for a polyglycine expansion in the N-terminal of the protein. It is associated with protein translation as a regulator of apoptosis signal-regulating kinase 1 (ASK1). GSPT1 interacts with ASK1 and enhances ASK1-induced apoptotic activity by activating caspase-3. It plays a novel role in the regulation of ASK1-mediated apoptosis. GSPT1 is found to be up-regulated in various human cancers and is considered as a potential proto-oncogene.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
106K4879

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Mahboobe Miri et al.
Medical oncology (Northwood, London, England), 29(3), 1581-1585 (2011-11-22)
The significance of translation regulatory factors in elevating the risk of cancer has been recently recognized. Eukaryotic release factor 3a (eRF3a) is a translation termination protein that is encoded by G1 to S phase transition 1 gene (GSPT1). The eRF3a/GSPT1
Joana Malta-Vacas et al.
Cancer genetics and cytogenetics, 195(2), 132-142 (2009-12-08)
The human eukaryotic release factor 3a (eRF3a), encoded by the G1 to S phase transition 1 gene (GSPT1; alias eRF3a), is upregulated in various human cancers. GSPT1 contains a GGC(n) polymorphism in exon 1, encoding a polyglycine expansion in the
J A Lee et al.
Oncogene, 27(9), 1297-1305 (2007-08-19)
Apoptosis signal-regulating kinase 1 (ASK1), a member of the mitogen-activated protein kinase kinase kinase family, plays a critical role in mediating apoptosis signals initiated by a variety of death stimuli such as hydrogen peroxide and tumor necrosis factor-alpha. Owing to

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