E3906
Anti-Early Endosomal Antigen 1 (C-terminal) antibody produced in rabbit
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
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Synonym(s):
Anti-EEA1, Anti-Endosome-associated Protein p162, Anti-Zinc Finger FYVE Domain-containing Protein 2
Recommended Products
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~160 kDa
species reactivity
mouse, rat, human
concentration
~1 mg/mL
technique(s)
indirect immunofluorescence: 5-10 μg/mL using rat NRK cells
western blot (chemiluminescent): 0.1-0.2 μg/mL using whole extract of human HeLa cells
western blot (chemiluminescent): 0.2-0.4 μg/mL using whole extract of mouse NIH3T3 cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... EEA1(8411)
mouse ... Eea1(216238)
rat ... Eea1(314764)
General description
Anti-Early Endosomal Antigen 1 (C-terminal), is produced in rabbit using a synthetic peptide corresponding to amino acid residues 1391-1410 of human EEA1, conjugated to keyhole limpet hemocyanin (KLH), as immunogen. Early Endosomal Antigen 1 (EEA1) is a 162 kDa autoantigen, which encodes for around 1400 amino acid proteins. EEA1 is a dimer, which comprises extensive coiled-coil regions. At its C-terminus, it contains a cysteine-rich zinc-finger-like domain named FYVE domain. The gene Early Endosome Antigen 1 (EEA1) encodes for around 1400 amino acid proteins
Immunogen
synthetic peptide corresponding to amino acid residues 1391-1410 of human EEA1, conjugated to KLH. The corresponding sequence is identical in mouse.
Application
Anti-Early Endosomal Antigen 1 (C-terminal) antibody produced in rabbit has been used in:
- fluorescent staining
- confocal laser scanning microscopy
- immunoprecipitation
- indirect immunofluorescence microscopy
- fluorescence microscopy
- immunoblotting
- immunofluorescence.
Biochem/physiol Actions
Anti- Early Endosomal Antigen 1 (EEA1) may be used as an early endosome marker. The binding of EEA1 to phosphatidylinositol 3-phosphate (PtdIns3P) and Rab5-GTP is essential for the localization and function of EEA1 in endocytic membrane fusion. It is associated with subacute systemic lupus erythematosus that specifically localized to early endosomes.
The gene Early Endosome Antigen 1 (EEA1) encodes for around 1400 amino acid proteins. It is a peripheral membrane protein associated with the cytoplasmic side of early endosomes. EEA1 controls vesicle fusion during endocytosis. In neurons, it is involved in recycling of synaptic vesicles and neurotransmitter receptors. Patients with neurological deficits develop EEA1 autoantibodies. Mutation in EEA1 gene leads to susceptibility to diabetes in the Japanese population.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
related product
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Certificates of Analysis (COA)
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