Skip to Content
Merck
CN
All Photos(5)

Documents

Safety Information

E3406

Sigma-Aldrich

Anti-EB1 antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-APC binding protein EB1, Anti-End-Binding-1, Anti-Microtuble-associated protein RP/EB family member 1

Sign Into View Organizational & Contract Pricing
All Photos(5)

About This Item

MDL number:
MFCD01633308
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 35 kDa

species reactivity

rat, mouse, bovine, pig, canine, human

concentration

~1.5 mg/mL

technique(s)

immunoprecipitation (IP): 10-20 μg using cell lysate of the human epitheloid carcinoma HeLa cell line
indirect immunofluorescence: 1-2 μg/mL using rat fibroblast Rat1 cells
indirect immunofluorescence: suitable
western blot: 0.5-1 μg/mL using cell lysate of the rat fibroblast cell line Rat1 or Rat 2

UniProt accession no.

Q15691

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MAPRE1(22919)
mouse ... Mapre1(13589)
rat ... Mapre1(114764)

General description

Microtubule-associated protein RP/EB family member 1 is a protein encoded by the MAPRE1 gene in humans. EB1 (End-Binding-1) proteins belong to a distinct family of accessory proteins, termed “plus-end tracking proteins” or +TIPs, that bind to the plus-ends of microtubules. All EB1 family proteins have a highly conserved N-terminal region that contains a calponin homology (CH) domain and an EB1-like C-terminal motif, which is unique to EB1 family proteins.

Immunogen

synthetic peptide corresponding to amino acids 252-268 located at the C-terminus of human EB1, conjugated to KLH. This sequence is identical in several species (mouse, rat, dog, bovine, and porcine EB1), highly conserved (single amino acid substitution) in chicken EB1, and has limited homology to human EB2 (35% identity) and human EB3 (53% identity).

Application

Anti-EB1 antibody produced in rabbit has been used in
  • immunofluorescence
  • western blotting
  • immunocytochemistry
  • immunoprecipitation

Biochem/physiol Actions

End-Binding-1 (EB1) associates with components of the dynactin complex and cytoplasmic dynein intermediate chain.
The C-terminal domain of End-binding proteins (EBs) is important for its autonomous microtubule tip tracking, regulation of microtubule dynamics, chromosome segregation and recruitment of numerous partners to microtubule ends. EB1 proteins bind to microtubule ends and act with the adenomatous polyposis coli (APC) tumor suppressor to regulate the microtubule filament system. It is a potential oncogene and plays an important role during early event in colon carcinogenesis, colorectal cancer (CRC). EB1 acts as a potential biomarker and therapeutic target in CRC.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

EB1 contributes to proper front-to-back polarity in neutrophil-like HL-60 cells
Samereier M, et al.
European Journal of Cell Biology, 96(2), 143-153 (2017)
EB1 proteins regulate microtubule dynamics, cell polarity, and chromosome stability
Tirnauer JS and Bierer BE
The Journal of cell biology, 149(4), 761-766 (2000)
Microtubule plus-end tracking proteins in neuronal development
van de Willige D, et al.
Cellular and Molecular Life Sciences, 73(10), 2053-2077 (2016)
The Leber congenital amaurosis protein AIPL1 and EB proteins co-localize at the photoreceptor cilium
Hidalgo-de-Quintana J, et al.
PLoS ONE, 10(3), e0121440-e0121440 (2015)
Characterization of functional domains of human EB1 family proteins
Bu W and Su LK
The Journal of biological chemistry, 278(50), 49721-49731 (2003)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service