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E3403

Millipore

EZview™ Red Protein G Affinity Gel

Synonym(s):

EZview Affinity Gel, Protein G Affinity Gel

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About This Item

MDL number:
MFCD03456743
UNSPSC Code:
12352202
NACRES:
NA.56

form

liquid

Quality Level

200

analyte chemical class(es)

proteins (Immunoglobulins of various mammalian species)

technique(s)

affinity chromatography: suitable
immunoprecipitation (IP): suitable

capacity

≥8 mg/mL, gel binding capacity (rabbit IgG)

storage temp.

−20°C

Related Categories

General description

EZ view Red Protein G Affinity Gel is Protein G covalently bonded to 4% Agarose beads. The affinity gel is used to bind the FC portion of the IgG of an antibody to samples, such as cell lysates and tissue, for purification of proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results.

Application

When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Features and Benefits

  • Increased visibility - Red color reduces risk of incidental aspiration
  • Improved recovery of target protein by reduced accidental loss
  • Higher reproducibility - More consistent yields

Physical form

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon
50% slurry in PBS with 50%Glycerol and 0.0015%

Legal Information

EZview is a trademark of Sigma-Aldrich Co. LLC

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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