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Merck
CN

E3138

Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse

clone F4, ascites fluid

Synonym(s):

Anti-EGFR

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About This Item

UNSPSC Code:
51111800
NACRES:
NA.41
MDL number:
MFCD00162282

Key Documents

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Product Name

Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse, clone F4, ascites fluid

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

F4, monoclonal

contains

15 mM sodium azide

species reactivity

human

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:2,000

isotype

IgG1

UniProt accession no.

P00533

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... EGFR(1956)

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Application

Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse has been used in:
  • western blotting
  • immunoprecipitation
  • immunocytochemistry
  • immunohistochemical staining

Biochem/physiol Actions

EGFR is a transmembrane glycoprotein that binds to the EGF family. It is a pivotal regulator of cell proliferation, growth, differentiation and DNA repair after radiation-induced damage. Mutations in the gene encoding EGFR are associated with a number of malignancies including lung cancer and colorectal cancer.
Epidermal growth factor receptor (EGF-R) is a tyrosine-specific protein kinase with the capacity to phosphorylate tyrosine residues located near its carboxy-terminus.
This antibody recognizes specifically an intracellular domain of the EGF receptor.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The receptor for epidermal growth factor is an integral cell membrane protein of 170 kD, which spans the membranes of a wide range of normal and malignant epithelial cells. EGF-R have been identified in sarcomas, gliomas, breast, bladder, lung and gynecological tumors. The EGF-R has a half-life of approximately 10 hours in human fibroblasts, but in the presence of EGF this value is reduced to about 1 hour.

Immunogen

synthetically produced residues 985-996 of the human EGF receptor.

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
0000482536
0000482536
0000482535
0000137615
0000092883

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Mechanisms of receptor tyrosine kinase activation in cancer
Du Z and Lovly CM
Molecular Cancer, 17(1), 58-58 (2018)
Netanya I Pollock et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 21(20), 4597-4606 (2015-07-04)
In other cancer types, HPV infection has been reported to coincide with overexpression of HER2 (ERBB2) and HER3 (ERBB3); however, the association between HER2 or HER3 expression and dimer formation in HNSCC has not been reported. Overexpression of HER2 and
M S Berger et al.
The Journal of pathology, 152(4), 297-307 (1987-08-01)
Immunocytochemical analysis of epidermal growth factor (EGF) receptor expression was carried out on frozen sections of 109 primary lung tumours resected at the Brompton Hospital from February 1984 to May 1985. Tumours with detectable levels of this proto-oncogene protein were
Qing Cheng et al.
EJNMMI research, 6(1), 58-58 (2016-07-09)
Though overexpression of epidermal growth factor receptor (EGFR) in several forms of cancer is considered to be an important prognostic biomarker related to poor prognosis, clear correlations between biomarker assays and patient management have been difficult to establish. Here, we
A Argiris et al.
Annals of oncology : official journal of the European Society for Medical Oncology, 24(1), 220-225 (2012-08-18)
We evaluated combined targeting with cetuximab, an anti-epidermal growth factor receptor (EGFR) monoclonal antibody, and bevacizumab, an anti-vascular endothelial growth factor (VEGF) monoclonal antibody, in squamous cell carcinoma of the head and neck (SCCHN). The combination was studied in human
View All Related Papers

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Data Sheet - E3138

Datasheet

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