E3138
Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse
clone F4, ascites fluid
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Synonym(s):
Anti-EGFR
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
F4, monoclonal
contains
15 mM sodium azide
species reactivity
human
packaging
antibody small pack of 25 μL
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:2,000
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... EGFR(1956)
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General description
Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The receptor for epidermal growth factor is an integral cell membrane protein of 170 kD, which spans the membranes of a wide range of normal and malignant epithelial cells. EGF-R have been identified in sarcomas, gliomas, breast, bladder, lung and gynecological tumors. The EGF-R has a half-life of approximately 10 hours in human fibroblasts, but in the presence of EGF this value is reduced to about 1 hour.
Specificity
This antibody recognizes specifically an intracellular domain of the EGF receptor.
Immunogen
synthetically produced residues 985-996 of the human EGF receptor.
Application
Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse has been used in:
- western blotting
- immunoprecipitation
- immunocytochemistry
- immunohistochemical staining
Biochem/physiol Actions
EGFR is a transmembrane glycoprotein that binds to the EGF family. It is a pivotal regulator of cell proliferation, growth, differentiation and DNA repair after radiation-induced damage. Mutations in the gene encoding EGFR are associated with a number of malignancies including lung cancer and colorectal cancer.
Epidermal growth factor receptor (EGF-R) is a tyrosine-specific protein kinase with the capacity to phosphorylate tyrosine residues located near its carboxy-terminus.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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A Argiris et al.
Annals of oncology : official journal of the European Society for Medical Oncology, 24(1), 220-225 (2012-08-18)
We evaluated combined targeting with cetuximab, an anti-epidermal growth factor receptor (EGFR) monoclonal antibody, and bevacizumab, an anti-vascular endothelial growth factor (VEGF) monoclonal antibody, in squamous cell carcinoma of the head and neck (SCCHN). The combination was studied in human
Qing Cheng et al.
EJNMMI research, 6(1), 58-58 (2016-07-09)
Though overexpression of epidermal growth factor receptor (EGFR) in several forms of cancer is considered to be an important prognostic biomarker related to poor prognosis, clear correlations between biomarker assays and patient management have been difficult to establish. Here, we
Changes in expression of epidermal growth factor receptors by anterior pituitary cells during the estrous cycle: cyclic expression by gonadotropes
Armstrong J and Childs GV
Endocrinology, 138(5), 1903-1908 (1997)
Localization of the epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) in the bovine testis
Kassab M, et al.
Journal of Molecular Histology, 38(3), 207-214 (2007)
T Cerny et al.
British journal of cancer, 54(2), 265-269 (1986-08-01)
Epidermal growth factor receptor (EGF-R) expression was assessed in 63 lung tumour samples with a monoclonal antibody (EGF-R1) by indirect immunoperoxidase staining on cryostat sections. All 15 small cell lung cancer samples were negative whereas over 80% of the 48
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