E3024
Earle′s Balanced Salts
With sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture
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Synonym(s):
EBSS
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Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
phenol red: no
NaHCO3: 2.2 g/L
glucose: 1.0 g/L (Dextro)
shipped in
ambient
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General description
Earle′s balanced salts solution (EBSS) is a saline solution with physiological pH that stimulates autophagy. It augments Bacillus Calmette-Guérin (BCG)-induced dendritic cells (DCs) by upregulating cluster of differentiation (CD86), human leukocyte antigen-DR isotype (HLA-DR), and interleukin (IL-6).
Application
Earle′s balanced salts has been used to incubate filter-grown monolayers of Caco-2 cells to induce starvation, along with HEPES buffer to detect NADPH oxidase activity in trypsinized cells. It has also been used to treat CEM RES cells and perform amino acid depletion experiment.
Other Notes
Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when working with stem cells or when growing cells at low densities.
supplement
Product No.
Description
Pricing
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Morgan Wong et al.
American journal of physiology. Cell physiology, 316(5), C753-C765 (2019-03-21)
A defective tight junction (TJ) barrier is a key pathogenic factor for inflammatory bowel disease. Previously, we have shown that autophagy, a cell survival mechanism, enhances intestinal epithelial TJ barrier function. Autophagy-related protein-6 (ATG6/beclin 1), a key protein in the
Natthakan Thongon et al.
Cancer & metabolism, 6, 1-1 (2018-03-16)
Inhibitors of nicotinamide phosphoribosyltransferase (NAMPT), the rate-limiting enzyme in NAD+ biosynthesis from nicotinamide, exhibit anticancer effects in preclinical models. However, continuous exposure to NAMPT inhibitors, such as FK866, can induce acquired resistance. We developed FK866-resistant CCRF-CEM (T cell acute lymphoblastic
Joon Hee Kang et al.
Biomolecules & therapeutics, 27(1), 34-40 (2018-09-21)
Transglutaminase 2 (TGase 2) plays a key role in p53 regulation, depleting p53 tumor suppressor through autophagy in renal cell carcinoma. We found that microtubule-associated protein 1A/1B-light chain 3 (LC3), a hallmark of autophagy, were tightly associated with the level
Jagadeesan Arunkumar et al.
Microbial pathogenesis, 118, 301-309 (2018-04-01)
Herpes simplex virus - 2 (HSV-2) causes lifelong persisting infection in the immunocompromised host and intermittent in healthy individuals with high morbidity in neonatals and also increase the transmission of HIV. Acyclovir is widely used drug to treat HSV-2 infection
Stanislas Morand et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 23(4), 1205-1218 (2008-12-17)
Dual oxidases (Duox1 and Duox2) are plasma membrane-targeted hydrogen peroxide generators that support extracellular hemoperoxidases. Duox activator 2 (Duoxa2), initially described as an endoplasmic reticulum resident protein, functions as a maturation factor needed to deliver active Duox2 to the cell
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