E2886
ExtrAvidin®−Peroxidase
buffered aqueous solution
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conjugate
peroxidase conjugate
Quality Level
form
buffered aqueous solution
technique(s)
direct ELISA: 1:2,000
dot blot: 1:4,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
shipped in
wet ice
storage temp.
2-8°C
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Specificity
The product is found to be reactive with Anti-Avidin, Catalog Number A5170, and non-reactive with Anti-Streptavidin, Catalog Number S6390, by immunoelectrophoresis.
Application
ExtrAvidin®-Peroxidase is suitable for the following applications:
- Dot blot at a minimum dilution of 1:4,000
- Immunohistochemistry (using formalin-fixed, paraffin-embedded sections at a dilution of 1:100)
- Direct ELISA at a minimum dilution of 1:2,000
- Immunoprecipitation and Immunobloting
- Ra2I solid-phase binding assays
- Southwestern blotting
- Western blotting
- Immunocytochemistry
Biochem/physiol Actions
Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin-Peroxidase may be used in conjunction with biotin in different techniques.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT
Preparation Note
Affinity purified protein
Storage and Stability
Store at 2-8 °C. Do Not Freeze. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Legal Information
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Hazard Classifications
Skin Sens. 1
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
动植物源性产品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
L Y Bourguignon et al.
The Journal of biological chemistry, 272(44), 27913-27918 (1997-11-05)
In this study we have examined the interaction between CD44s (the standard form) and the p185(HER2) proto-oncogene in the ovarian carcinoma cell line. Surface biotinylation followed by wheat germ agglutinin column chromatography and anti-CD44-mediated immunoprecipitation indicate that both CD44s and
G Grouard et al.
The Journal of experimental medicine, 185(6), 1101-1111 (1997-03-17)
A subset of CD4+CD11c-CD3- blood cells was recently shown to develop into dendritic cells when cultured with monocyte conditioned medium. Here, we demonstrate that CD4+ CD11c-CD3- cells, isolated from tonsils, correspond to the so-called plasmacytoid T cells, an obscure cell
S Correia et al.
Molecular human reproduction, 19(9), 581-589 (2013-04-26)
Sperm undergo maturation acquiring progressive motility and the ability to fertilize oocytes through exposure to the components of the epididymal fluid (EF). Although the establishment of a calcium (Ca(2+)) gradient along the epididymis has been described, its direct effects on
Peter Hauff et al.
Radiology, 231(3), 667-673 (2004-05-01)
To evaluate the feasibility of using intravenously administered L-selectin ligand-specific polymer-stabilized air-filled microparticles (MPs) for active targeting of peripheral lymph nodes under normal conditions in animal models. L-selectin ligand-specific MPs and two control substances (immunoglobulin M-isotype MPs and native MPs)
M C Dieu-Nosjean et al.
The Journal of experimental medicine, 192(5), 705-718 (2000-09-07)
Dendritic cells (DCs) form a network comprising different populations that initiate and differentially regulate immune responses. Langerhans cells (LCs) represent a unique population of DCs colonizing epithelium, and we present here observations suggesting that macrophage inflammatory protein (MIP)-3alpha plays a
Protocols
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
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