E2760
Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse
clone 29.1, ascites fluid
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Synonym(s):
Anti-ERBB, Anti-ERBB1, Anti-ERRP, Anti-HER1, Anti-NISBD2, Anti-PIG61, Anti-mENA
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
29.1, monoclonal
contains
15 mM sodium azide
species reactivity
pig, human, rat, mouse, canine
should not react with
rabbit
technique(s)
indirect ELISA: 1:10,000 using extract of the A431 cell line
western blot: 1:3,000 using extract of the A431 cell line
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egfr(24329)
Related Categories
General description
Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The receptor for epidermal growth factor is an integral cell membrane protein of 170 kDa, which spans the membranes of a wide range of normal and malignant epithelial cells. A close similarity has been found between the sequence of the avian erythroblastic leukemia viral oncogene homolog (v-erb-B) and the cytoplasmic and transmembrane part of the EGF-R (truncated EGF-R).
Specificity
Localizes blood type A-related determinants on various glycoproteins and glycolipids in addition to specific EGF receptor determinants (only observed in human tissue). Binds an external carbohydrate on the receptor and does not block EGF binding.
Immunogen
human carcinoma cell line A431, which expresses high levels of EGF receptors.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
May be used to purify EGF receptor, for structural and functional studies of the receptor and for investigation of cell transformation versus oncogenesis.
Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse has been used in
- enzyme linked immunosorbent assay (ELISA)
- immunoprecipitation
- immunoaffinity
- immunohistochemistry
- western blotting
Biochem/physiol Actions
The members of epidermal growth factor receptor (EGF R) or the ErbB receptor family have been identified as useful biomarkers and targets for cancer therapy. The EGFR family includes four receptor tyrosine kinases, EGF R (ErbB1), ErbB2 (Neu), ErbB3, and ErbB4. EGF R binds EGF and induces tyrosine phosphorylation leading to proliferation of cells. EGF R is present on many cell types of epithelial and mesenchymal lineages. EGF R is capable of binding transforming growth factor-α and heparin-binding EGF in addition to EGF. There are numerous effector molecule activated by EGF R that result in a variety of biological processes such as morphogenesis, cell motility, apoptosis, differentiation and organ repair and maintenance. Deregulation of EGF R signaling is implicated in progression of a wide variety of tumors, invasion and metastasis.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
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Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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B Assarsson et al.
Acta physiologica Scandinavica, 154(2), 177-183 (1995-06-01)
Members of the epidermal growth factor (EGF) family have many different effects on ovarian cells including a strong inhibition of estradiol-17 beta production. An increased binding of the EGF family to its receptor is seen at the time of ovulation.
Epidermal growth factor receptor in relation to tumor development: EGFR gene and cancer
Mitsudomi T and Yatabe Y
The FEBS journal, 277(2), 301-308 (2010)
Hong Suk Song et al.
Cancer research and treatment : official journal of Korean Cancer Association, 36(4), 240-245 (2004-08-01)
The purpose of this study was to investigate the prognostic significance of the expression of EGFR and C-erbB-2 gene products by immunohistochemical analysis for curatively resected gastric adenocarcinoma. Between January 1996 and December 2001, 739 patients with curatively resected gastric
Nicole Salazar et al.
Molecular cancer, 13, 198-198 (2014-08-30)
Recent advances have revealed a significant contribution of chemokines and their receptors in tumor growth, survival after chemotherapy, and organ-specific metastasis. The CXC chemokine receptor-7 (CXCR7) is the latest chemokine receptor implicated in cancer. Although over expressed in breast cancer
David J Javier et al.
Journal of nanophotonics, 2(1), 23506-23506 (2008-12-11)
Anisotropic metal-based nanomaterials have been proposed as potential contrast agents due to their strong surface plasmon resonance. We evaluated the contrast properties of gold, silver, and gold-silver hybrid nanorods for molecular imaging applications in three-dimensional biological samples. We used diffuse
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