E2264
Endoglycosidase F3 from Elizabethkingia miricola
recombinant, expressed in E. coli, 30 U/mg
Synonym(s):
Elizabethkingia miricola, Endo-β-N-acetylglucosaminidase F3, Endoglycosidase F3 from Elizabethkingia (Chryseobacterium/Flavobacterium) meningosepticum
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About This Item
Specific activity:
30 U/mg
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
Quality Segment
conjugate
(N-linked)
form
solution
specific activity
30 U/mg
mol wt
32 kDa
shipped in
wet ice
storage temp.
2-8°C
Application
Endoglycosidase F3 from Elizabethkingia miricola has been used to analyze core fucosylation and tryptic digests of serum proteins.
Biochem/physiol Actions
Endoglycosidase F3 belongs to the glycoside hydrolase family 18 (GH18). It has hydrolytic activity. Endoglycosidase F3 glycosylates α-1,6-fucosylated GlcNAc derivative to give natural, core fucosylated complex-type N-glycopeptides.
Cleaves asparagine-linked biantennary and triantennary complex, oligosaccharides depending on the state of core fucosylation and peptide linkage.
Packaging
Supplied with 5× Reaction Buffer, 250 mM sodium acetate, pH 4.5
Physical form
Aseptically filled solution in 20 mM Tris-HCl, pH 7.5
Other Notes
One unit will release N-linked oligosaccharides from 1 μmole of denatured porcine fibrinogen in 1 minute at 37 °C, pH 4.5.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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