E2145
Express-Ion™ exchanger D free base
60-130 μm
Synonym(s):
DEAE-Cellulose, Cellulose DEAE, Diethylaminoethyl-cellulose
InChI
1S/C12H22O11/c13-1-3-5(15)6(16)9(19)12(22-3)23-10-4(2-14)21-11(20)8(18)7(10)17/h3-20H,1-2H2/t3?,4?,5?,6?,7?,8?,9?,10-,11?,12+/m1/s1
SMILES string
OC[C@@H]1O[C@@H](O[C@H]2[C@@H](O)[C@H](O)[C@@H](O)O[C@H]2CO)[C@@H](O)[C@H](O)[C@H]1O
InChI key
GUBGYTABKSRVRQ-WFVLMXAXSA-N
matrix active group
DEAE (diethylaminoethyl)
particle size
60-130 μm
pH
2-9
capacity
1 meq/g
Application
Express-Ion exchangers are weakly basic anion exchangers used in protein chromatography, ion exchange chromatography, cation exchange media, cellulose ion exchange TLC adsorbents and DEAE-cellulose anion exchange TLC adsorbents.
Legal Information
Express-Ion is a trademark of Whatman International Ltd.
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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G Denton et al.
Journal of chromatography. A, 908(1-2), 223-234 (2001-02-24)
The chromatography of the murine hybridoma cell C595/102 culture supernatant expressing the therapeutic monoclonal antibody C595, on the cation-exchange cellulose Whatman Express-Ion Exchanger S has been investigated. Initial method scouting studies using purified C595 in 1-ml mini columns demonstrated that
Direct enzyme adsorption from an unclarified microbial feedstock using suspended bed chromatography.
Tau Chuan Ling et al.
Journal of chromatography. A, 989(1), 109-118 (2003-03-19)
Suspended bed chromatography (SBC) is a new hybrid technique concomitantly benefiting from batch adsorption, the process advantages of an enclosed system, and its compatibility with established commercial chromatographic contactors and adsorbents. SBC was evaluated in the anion-exchange capture and chromatographic
P R Levison et al.
Journal of chromatography. A, 890(1), 45-51 (2000-09-08)
A new technique in downstream processing, suspended bed chromatography has been developed. This hybrid technique exploiting the benefits of batch adsorption and the process advantages of an enclosed column system can be carried out using established contactors and adsorbents. A
Jack H Wong et al.
Journal of the science of food and agriculture, 90(1), 70-77 (2010-04-01)
A haemagglutinin was purified from Japanese Hokkaido red beans (Phaseolus vulgaris cv. Hokkaido red bean) with a procedure that included three chromatographic media. Haemagglutinating activity was adsorbed on DEAE cellulose, Affi-gel blue gel and Mono S. The pure haemagglutinin was
Tiago Vicente et al.
Biotechnology and bioengineering, 108(6), 1347-1359 (2011-02-05)
The effect of ligand density on anion-exchange membrane chromatography (AEXmc) for the purification of recombinant baculoviruses (rBVs), potential viral vectors in clinical applications, is studied by surface plasmon resonance on customized AEX surfaces and gradient elution experiments on Sartobind D
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