DNB600
GenElute™ Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit
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General description
Sigma′s GenElute™ Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit provides a fast, reliable and simple procedures for isolating cell-free circulating DNA (cfc-DNA) from various amounts of plasma/serum ranging from 1 mL up to 4 mL. Purification is based on spin column chromatography that uses Sigma′s proprietary resin separation matrix. The kits are designed to isolate all sizes of cfc-DNA from either fresh or frozen plasma/serum samples. Moreover, these kits allow the user to elute the purified cfc-DNA into a flexible elution volume ranging from 25 μL to 50 μL. The purified plasma/serum cfc-DNA is eluted in an Elution Buffer that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.
- Isolate all sizes of circulating DNA from plasma and serum samples
- Isolate viral and bacterial DNA
- Versatile plasma and serum input volumes (1 mL – 4 mL)
- Concentrate circulating DNA into a flexible elution volume ranging from (50 μL - 100 μL)
- Isolate inhibitor-free cell-free circulating DNA
- Purify high-quality DNA in 40-45 minutes
- Compatible with Streck Cell-Free DNA BCT Tubes
Application
- PCR
- qPCR
- Southern Blot
- Methylation-sensitive PCR
- CpG array
- Restriction enzyme digestions
- Viral Detection
- Bacterial Detection
- Microarray
- NGS
Unit Definition
Preps
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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Danger
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Precautionary Statements
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2
WGK
WGK 3
Regulatory Information
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