D9945
Anti-DOCK1 (C-terminal) antibody produced in rabbit
~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-DOCK180, Anti-Dedicator of cytokinesis 1, Anti-Downstream of CRK-binding protein, 180-KD
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, WB
Citations:
4
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~180 kDa
species reactivity
mouse, rat, human
concentration
~1.5 mg/mL
technique(s)
indirect immunofluorescence: 10-20 μg/mL using NIH-3T3 cells, western blot: 2-4 μg/mL using HeLa or PC12 cell lysates
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... DOCK1(1793)
mouse ... Dock1(330662)
rat ... Dock1(309081)
General description
Dedicator of cytokinesis protein 1 (DOCK1), also termed as the DOCK180 gene, is mapped to human chromosome 10q26.2. DOCK1 is a member of a family of guanine nucleotide exchange factors (GEF).
Application
Anti-DOCK1 (C-terminal) antibody produced in rabbit may be used in immunoblotting and immunofluorescence.
Biochem/physiol Actions
Anti-DOCK1 (C-terminal) specifically recognizes human, rat, and mouse DOCK1.
Dedicator of cytokinesis protein 1 (DOCK1) is involved in cytoskeletal reorganization and cell migration and acts as an activator of Rac. DOCK1 has been found to undergo ubiquitination and proteasome-dependent degradation, a process enhanced in response to epidermal growth factor (EGF), adapter molecule (Crk) and adhesion-dependent signals. DOCK1 forms a complex with engulfment and cell motility protein 1 (ELMO1) which also inhibits ubiquitylation of DOCK1. By activating the β1 and αvβ5 integrins, DOCK1 regulates cell motility, filopodia formation, and phagocytosis. DOCK1 has been suggested to play a central role in netrin signaling during neuronal development by mediating the attractive responses by neurons to netrin-1. It mediates morphological changes such as integrin-mediated cell spreading and nerve growth factor (NGF)-induced neurite outgrowth. In mammalian cells, the CrkII-ELMO1-DOCK1 complex has been shown to promote phagocytosis.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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M L Albert et al.
Nature cell biology, 2(12), 899-905 (2001-01-09)
Integrin receptors are important for the phagocytosis of apoptotic cells. However, little is known about their function in mediating internalization, as previous studies used blocking antibodies for the inhibition of binding. Here we show that the alphavbeta5 receptor mediates both
Rapid aneuploidy diagnosis of partial trisomy 7q (7q34? qter) and partial monosomy 10q (10q26. 12? qter) by array comparative genomic hybridization using uncultured amniocytes
Chen CP, et al.
Taiwanese Journal of Obstetrics & Gynecology, 51(1), 93-99 (2012)
Xiaoling Li et al.
Nature neuroscience, 11(1), 28-35 (2007-12-11)
Netrins are prototypical axon guidance cues whose attractive signaling requires the small GTPase Rac1. It remains unclear how Rac1 is regulated in the netrin pathway. DOCK180 is a member of a new family of guanine nucleotide exchange factors for Rho
Yoshinori Makino et al.
Journal of cell science, 119(Pt 5), 923-932 (2006-02-24)
Dock180, a member of the CDM family of proteins, plays roles in biological processes such as phagocytosis and motility through its association with the signalling adaptor protein Crk. Recently, the complex formation between Dock180 and Elmo1 was reported to function
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