D9692
Plant Protoplast Digest/Wash Solution
Rapid isolation of viable protoplasts from plant tissue
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About This Item
UNSPSC Code:
41105500
NACRES:
NA.56
General description
The Plant Protoplast Digest/Wash Solution is formulated to facilitate the rapid isolation of viable protoplasts from plant tissue. Plant cells are surrounded by a rigid, semi-permeable cell wall composed primarily of three classes of polysaccharides: cellulose, hemicellulose, and pectin. The Plant Protoplast Digest/Wash Solution can be used for the digestion of the cell wall after the addition of enzymes that hydrolyze these polysaccharides, such as cellulase, pectinase, or pectolyase. The Plant Protoplast Digest/Wash Solution can be subsequently used to wash away any remaining hydrolytic enzymes after digestion is complete.
Plant protoplasts are typically used for any of a number of downstream applications. These applications include, but are not limited to, transient gene expression, viral transfection assays, somatic hybridization, electrophysiological studies, and morphological studies.
Plant protoplasts are typically used for any of a number of downstream applications. These applications include, but are not limited to, transient gene expression, viral transfection assays, somatic hybridization, electrophysiological studies, and morphological studies.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Shiu-Cheung Lung et al.
Plant cell reports, 30(4), 473-484 (2010-11-26)
Although transient gene expression using reporters such as green fluorescent protein is a versatile tool for examining gene functions and intracellular protein trafficking, the establishment of a highly efficient gene manipulation method remains a challenge in many plant species. A
Advanced patch-clamp techniques and single-channel analysis.
White, P. J. et al
The Journal of Experimental Biology, 50, 1037-1054 (1999)
Vera Bandmann et al.
Molecular plant, 4(2), 241-251 (2010-12-08)
To analyze the kinetics and size of single exo- and endocytotic events in BY-2 protoplasts, we employed cell-attached membrane capacitance measurements. These measurements revealed different modes of fusion and fission of single vesicles. In about half of the observed exocytotic
The cell wall.
Carpita, N., and McCann, M. et al.
Biochemistry, 52-108 null
Akira Inoue et al.
Marine biotechnology (New York, N.Y.), 13(2), 256-263 (2010-04-16)
Functional recombinant abalone alginate lyase (rHdAly) and β-1,4-endoglucanase (rHdEG66) were expressed as secreted proteins with baculoviral expression systems. The specific activity of each recombinant enzyme, 2,490 and 18.2 U/mg for rHdAly and rHdEG66, respectively, was comparable to its native form
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