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D9655

Sigma-Aldrich

pBR322 Hae III Digest

for DNA electrophoresis

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.25

Quality Level

100

form

liquid

usage

1000 uses (0.5 mg size)
200 uses (0.1 mg size)

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

Related Categories

General description

Sigma′s pBR322 Hae III Digest contains 22 fragments from 8 - 587 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Application

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Components

Sigma′s pBR322 Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains 22 fragments, 8–587 bp
For optimal resolution, the recommended agarose gel concentration is 2.5.

Preparation Note

Plasmid pBR322 DNA was digested with Hae III to completion, then purified by phenol extraction.

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Articles

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

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