D8783
7-Deaza-2′-deoxyguanosine 5′-triphosphate lithium salt
10 mM in H2O
Synonym(s):
−N7-dGTP, 7-Deaza-dGTP
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About This Item
Empirical Formula (Hill Notation):
C11H17N4O13P3
CAS Number:
Molecular Weight:
506.19
NACRES:
NA.52
PubChem Substance ID:
UNSPSC Code:
41106305
MDL number:
Assay:
≥95% (HPLC)
Biological source:
Porcine muscle, rabbit muscle
Form:
liquid
Storage temp.:
−20°C
InChI
1S/C11H17N4O13P3/c12-11-13-9-5(10(17)14-11)1-2-15(9)8-3-6(16)7(26-8)4-25-30(21,22)28-31(23,24)27-29(18,19)20/h1-2,6-8,16H,3-4H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,12,13,14,17)
SMILES string
NC1=NC(=O)c2ccn(C3CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O3)c2N1
InChI key
DLLXAZJTLIUPAI-UHFFFAOYSA-N
biological source
Porcine muscle, rabbit muscle
assay
≥95% (HPLC)
form
liquid
Quality Level
feature
PCR Additives
concentration
10 mM in H2O
technique(s)
PCR: suitable
color
colorless
shipped in
dry ice
storage temp.
−20°C
Related Categories
Application
7-Deaza-2′-deoxyguanosine 5′-triphosphate lithium salt has been used as a lysis agent to evaluate its efficiency on direct cell lysis, RNA stability, and compatibility with downstream reverse transcription quantitative real-time PCR during the analyzes of samples with small numbers of cells.
Biochem/physiol Actions
7-Deaza-2′-deoxyguanosine 5′-triphosphate (7-deaza-dGTP) competes with natural substrate dGTP and blocks the telomerase activity. This nucleotide once incorporated into the telomere alters the secondary structure of the telomere making it difficult for telomerase to identify it for further telomere synthesis. This dGTP analog pairs weakly with conventional bases to minimize DNA secondary structures, while being a good substrate for DNA polymerases. 7-deaza-dGTP in combination with dimethyl sulfoxide (DMSO) and betaine enhances the polymerase chain reaction (PCR) amplification of GC-rich DNA sequences.
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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