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D8515

Sigma-Aldrich

Deoxyribonucleic acid−cellulose double-stranded from calf thymus DNA

lyophilized powder

Synonym(s):

DNA, Deoxyribonucleic acid, ds-DNA-Cellulose, DNA-Cellulose

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About This Item

MDL number:
MFCD00130925
UNSPSC Code:
41106305
eCl@ss:
32160414
NACRES:
NA.51

form

lyophilized powder

Quality Level

200

extent of labeling

3-8 mg double-stranded calf thymus DNA/g

storage temp.

−20°C

General description

Deoxyribonucleic acid−cellulose double-stranded from calf thymus DNA, also known as ctDNA is a natural source of DNA designed for DNA binding agent studies. The detection limits of ctDNA are around 2.0ng and the linear ranges of light scattering intensity (I(LS)) is 0.05-1.5 mµg.

Application

Deoxyribonucleic acid−cellulose double-stranded from calf thymus DNA is used to study the binding parameters and binding specificity of a variety of proteins, nucleotides, and chemical reagents including anti-cancer agents. It is frequently used in pull-down assays and chromatographic procedures.

Quality

May contain a trace of Tris and EDTA.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物源性产品

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000314668
0000362206
0000357373
0000357374
0000362206

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If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What buffer do you use to swell the Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA (DNA Cellulose) resin, Product D8515?

    DNA-Cellulose can be swollen in 10 mM tris, 1 mM tetrasodium EDTA buffer (pH 7.9).  One gram of DNA-Cellulose will swell to approximately 4 mL of wet resin.

  4. How do you store Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA (DNA Cellulose) resin, Product D8515, once it is swollen?

    DNA-Cellulose can be stored swollen in 10 mM tris, 1 mM tetrasodium EDTA buffer (pH 7.5 - 8) for several months at 4°C (without a bacteriostat).

  5. Can Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA (DNA Cellulose), Product D8515, be exposed to high salt?

    DNA-Cellulose is stable in up to 5M NaCl.  DNA-Cellulose should not be exposed to solutions of NaOH, since NaOH denatures DNA from double-stranded to single-stranded.

  6. How do you regenerate the column using Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA (DNA Cellulose), Product D8515?

    A suggested procedure for the regeneration of a DNA-Cellulose column would be to use 5 - 10 column volumes of 2M NaCl in buffer, followed by the same volume of buffer alone.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. Can Product D8515, Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA, be used for purification of DNA-dependent RNA Polymerase?

    We have not tested Product D8515, Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA for this application. However, the following references utilizes double-stranded DNA cellulose for purification of DNA-dependent RNA Polymerase:J. of Bacteriology, 172, 5624-5630 (1990)Biochem. J.,133, 201-203 (1973)

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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