D8144
Dextranase from Penicillium sp.
lyophilized powder, 400-800 units/mg protein
Synonym(s):
1,6-α-D-Glucan 6-glucanohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-803-9
MDL number:
Product Name
Dextranase from Penicillium sp., lyophilized powder, 400-800 units/mg protein
biological source
Penicillium sp.
form
lyophilized powder
specific activity
400-800 units/mg protein
composition
Protein, 35% Lowry
storage temp.
2-8°C
Quality Level
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Application
Dextranase from Penicillium sp has been used:
- in the digestion of exopolysaccharides from P. aeruginosa biofilms and dextran polymers
- as a component of trypticase soy broth (TSYPS) for Streptococcus mutans culture
- in microgel degradation
Biochem/physiol Actions
An endodextranase that hydrolyzes -(1,6)-glucosidic linkages in dextran. Dextrans are undesirable compounds synthesized from sucrose by microbial contaminants during sugar production that increase viscosity of the flow and decrease industrial recovery. Dextranase has been used for hydrolyzing dextran at sugar mills in order to improve efficiency of sugar production.
General description
Dextranase from fungi belong to the glycoside hydrolase families (GH) 49. Dextranase from Penicillium sp. are stable at varying temperature and pH.
Other Notes
One unit will liberate 1.0 μmole of isomaltose (measured as maltose) per min at pH 6.0 at 37 °C, using dextran as substrate.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Biology and genome sequence of Streptococcus mutans phage M102AD
Delisle AL, et al.
Applied and Environmental Microbiology, 78(7), 2264-2271 (2012)
T I Danilova et al.
Prikladnaia biokhimiia i mikrobiologiia, 19(1), 104-110 (1983-01-01)
The carbohydrate composition of dextranase from Penicillium funiculosum 15, as well as the composition of products of dextran deep hydrolysis by the enzyme were studied. The products are normally used to stabilize the enzyme during its purification. Using the methods
Label-free high-throughput functional lytic assays
O'Malley SM, et al.
Journal of Biomolecular Screening, 12(1), 117-125 (2007)
Dextranase from Penicillium minioluteum: reaction course, crystal structure, and product complex
Larsson AM, et al.
Structure, 11(9), 1111-1121 (2003)
Hordenine: a novel quorum sensing inhibitor and antibiofilm agent against Pseudomonas aeruginosa
Zhou JW, et al.
Journal of Agricultural and Food Chemistry, 66(7), 1620-1628 (2018)
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